COVID 19 (BA.2.75) Spike S1+S2 trimer Coronavirus Active Protein | COVID-19 active protein

SARS-CoV-2 (BA.2.75) Spike S1+S2 trimer Protein (ECD, His Tag)

Cat. Num. AAA258580

• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-HPLC.
• Synonyms: COVID 19 (BA.2.75) Spike S1+S2 trimer Coronavirus; N/A; SARS-CoV-2 (BA.2.75) Spike S1+S2 trimer Protein (ECD, His Tag); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; (BA.2.75) Spike S1+S2 trimer; COVID-19 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE. >95% as determined by SEC-HPLC.
• Form/Format: Lyophilized from sterile 25mM Sodium Citrate, 200mM NaCl, 0.02%tween80, pH 6.0.
• Sequence: Met1-Gln1208

• Species: SARS-CoV-2
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Tag: C-His
• Predicted N Terminal: Val 16
• Protein Construction: A DNA sequence encoding the SARS-CoV-2 (BA.2.75) Spike S1+S2 (YP_009724390.1, with mutations T19I, L24del, P25del, P26del, A27S, G142D, K147E, W152R, F157L, I210V, V213G, G257S, G339H, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, N460K, S477N, T478K, E484A, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants) expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.2.75).
• Activity: Immobilized human ACE2 protein (mFc tag) at 2 ug/mL (100 uL/well) can bind Recombinant SARS-CoV-2 (BA.2.75) Spike S1+S2 trimer Protein (ECD, His Tag), the EC50 is 15-40 ng/mL.
• Preparation and Storage: Store it under sterile conditions at -20 to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Application Data

Application Data

(Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 ug/mL (100 uL/well) can bind Recombinant SARS-CoV-2 (BA.2.75) Spike S1+S2 trimer Protein (ECD, His Tag)(Cat:AAA258580), the EC50 is 15-40 ng/mL.)

Application Data

Related Product Information for COVID-19 active protein

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Product Categories/Family for COVID-19 active protein

coronavirus

NCBI and Uniprot Product Information

• Molecular Weight: 51.5 kDa