Bioactivity
(Immobilized Recombinant 2019-nCoV Spike RBD Protein at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.12-9.06 ng/mL.)
COVID 19 Spike RBD Coronavirus Active Protein | COVID-19 active protein
Recombinant 2019-nCoV Spike RBD Protein with His tag
Purity
> 95% by SDS-PAGE;> 95% by HPLC
Synonyms
COVID 19 Spike RBD Coronavirus; N/A; Recombinant 2019-nCoV Spike RBD Protein with His tag; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike RBD; Receptor Binding Domain; COVID-19 active protein
Host
HEK293 Cells
Purity/Purification
> 95% by SDS-PAGE;> 95% by HPLC
Form/Format
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4 or Supplied as a 0.22 um filtered solution in PBS, pH 7.4.
Sequence
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
Tag Information
6×His tag at the C-terminus
Endotoxin
< 0.1 EU/ug of the protein by LAL method.
Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV Spike RBD Protein at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.12-9.06 ng/mL.
Reconstitution
For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Preparation and Storage
Store the lyophilized protein at -20 degree C to -80 degree C for long term.|After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week. or This liquid product is stable at <= ?70 degree C for up to 1 year from the date of receipt.|For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
Bioactivity
(Immobilized Recombinant 2019-nCoV Spike RBD Protein at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.12-9.06 ng/mL.)
Bioactivity
(Immobilized Recombinant 2019-nCoV Spike RBD Protein at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.12-9.06 ng/mL.)
SDS-PAGE
(Immobilized Recombinant 2019-nCoV Spike RBD Protein at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.12-9.06 ng/mL.)
SDS-PAGE
(Immobilized Recombinant 2019-nCoV Spike RBD Protein at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.12-9.06 ng/mL.)
Related Product Information for COVID-19 active protein
Recombinant 2019-nCoV Spike RBD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.
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Product Notes
The COVID-19 (Catalog #AAA281535) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is 6×His tag at the C-terminus!!Endotoxin||< 0.1 EU/ug of the protein by LAL method. The amino acid sequence is listed below: RVQPTESIVR FPNITNLCPF GEVFNATRFA SVYAWNRKRI SNCVADYSVL YNSASFSTFK CYGVSPTKLN DLCFTNVYAD SFVIRGDEVR QIAPGQTGKI ADYNYKLPDD FTGCVIAWNS NNLDSKVGGN YNYLYRLFRK SNLKPFERDI STEIYQAGST PCNGVEGFNC YFPLQSYGFQ PTNGVGYQPY RVVVLSFELL HAPATVCGPK KSTNLVKNKC VNF. It is sometimes possible for the material contained within the vial of "COVID 19 Spike RBD Coronavirus, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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