Ephrin-B1 Active Protein | EFNB1 active protein

Ephrin-B1 Protein, Human, Recombinant (His & hFc Tag)

Cat. Num. AAA257947

• Gene Names: EFNB1; CFND; CFNS; EFL3; EPLG2; Elk-L; LERK2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Ephrin-B1; N/A; Ephrin-B1 Protein, Human, Recombinant (His & hFc Tag); Human Ephrin-B1/EFNB1 Protein (His & Fc Tag); CFND Protein, Human; CFNS Protein, Human; EFB1 Protein, Human; EFL3 Protein, Human; Elk-L Protein, Human; EPLG2 Protein, Human; LERK2 Protein, Human; ephrin-B1; EFNB1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys237

• Species: Human
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EphB3 at 2ug/ml (100uL/well) can bind human EFNB1 Fc chimera with a linear range of 1.56-25ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 28
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the human EFNB1 (NP_004420.1) extracellular domain (Met 1-Lys 237) was was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized mouse EphB3 at 2 ug/ml (100 ul/well) can bind human EFNB1 Fc chimera with a linear ranger of 1.56-25 ng/ml.)

SDS-PAGE

Related Product Information for EFNB1 active protein

Background: Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.

References

Davy A, et al. (2004) Ephrin-B1 forward and reverse signaling are required during mouse development. Genes Dev. 18(5): 572-83.Compagni A, et al. (2003) Control of skeletal patterning by ephrinB1-EphB interactions. Dev Cell. 5(2): 217-30.Wieland I, et al. (2004) Mutations of the ephrin-B1 gene cause craniofrontonasal syndrome. Am J Hum Genet. 74(6): 1209-15.

NCBI and Uniprot Product Information

• NCBI GI #: 4758248
• NCBI GeneID: 1947
• NCBI Accession #: NP_004420.1
• NCBI GenBank Nucleotide #: NM_004429.4
• UniProt Accession #: P98172; D3DVU0
• Molecular Weight: 38,007 Da
• NCBI Official Full Name: ephrin-B1
• NCBI Official Synonym Full Names: ephrin-B1
• NCBI Official Symbol: EFNB1
• NCBI Official Synonym Symbols: CFND; CFNS; EFL3; EPLG2; Elk-L; LERK2
• NCBI Protein Information: ephrin-B1; EFL-3; LERK-2; ELK ligand; ligand of eph-related kinase 2; eph-related receptor tyrosine kinase ligand 2
• UniProt Protein Name: Ephrin-B1
• UniProt Gene Name: EFNB1
• UniProt Synonym Gene Names: EFL3; EPLG2; LERK2; ELK-L; LERK-2
• UniProt Entry Name: EFNB1_HUMAN