Growth Hormone Receptor Active Protein | GHR active protein

Growth Hormone Receptor Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA258123

• Gene Names: Ghr; GHBP; GHR/BP
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Growth Hormone Receptor; N/A; Growth Hormone Receptor Protein, Mouse, Recombinant (His Tag); Mouse Growth Hormone Receptor/GHR/GHBP Protein (His Tag); GHBP Protein, Mouse; GHR/BP Protein, Mouse; growth hormone receptor; GHR active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln273

• Species: Mouse
• Activity: Measured by its ability to inhibit proliferation of INS-1 cells induced by human growth hormone. The ED50 for this effect is 2-8ug/mL in the presence of 50ng/mL human growth hormone.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse GHR (NP_034414.2)(Met 1-Gln 273) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured by its ability to inhibit proliferation of INS-1 cells induced by human growth hormone. The ED50 for this effect is 2-8ug/mL in the presence of 50 ng/mL human growth hormone.)

SDS-PAGE

Related Product Information for GHR active protein

Background: Growth hormone receptor, also known as GH receptor and GHR, is a single-pass type I membrane protein which belongs to thetype I cytokine receptor family and type 1 subfamily. GHR contains onefibronectin type-III domain. Growth hormone receptor/GHR is expressed in various tissues with high expression in liver and skeletal muscle. Isoform4of GHR is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform1 expression of GHR in placenta is predominant in chorion and decidua. Isoform4is highly expressed in placental villi. Isoform2of GHR is expressed in lung, stomach and muscle. Growth hormone receptor/GHR is a receptor for pituitary gland growth hormone. It is involved in regulating postnatal body growth. On ligand binding, it couples to the JAK2/STAT5 pathway. Isoform2of GHR up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. Defects in GHR are a cause of Laron syndrome (LARS) which is a severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may also be a cause of idiopathic short stature autosomal (ISSA) which is defined by a subnormal rate of growth.

References

Leung DW. et al., 1987, Nature. 330:537-43. Sobrier M-L. et al., 1997, J Clin Endocrinol Metab. 82: 435-7. Enberg B. et al., 2000, Eur J Endocrinol. 143: 71-6. Pantel J. et al., 2000, J Biol Chem. 275: 18664-9. Jorge AAL. et al., 2004, Clin Endocrinol. (Oxf.) 60: 36-40.

NCBI and Uniprot Product Information

• NCBI GI #: 114520600
• NCBI GeneID: 14600
• NCBI Accession #: NP_034414.2
• NCBI GenBank Nucleotide #: NM_010284.3
• Molecular Weight: 34,105 Da
• NCBI Official Full Name: growth hormone receptor isoform 1
• NCBI Official Synonym Full Names: growth hormone receptor
• NCBI Official Symbol: Ghr
• NCBI Official Synonym Symbols: GHBP; GHR/BP
• NCBI Protein Information: growth hormone receptor