IL-6 Receptor (IL-6R) Active Protein | CD126 active protein
Human IL-6 Receptor Recombinant (IL-6R)
Purity
>97%, as determined by SDS-PAGE and HPLC
Synonyms
IL-6 Receptor (IL-6R); N/A; Human IL-6 Receptor Recombinant (IL-6R); Human CD126 Recombinant (IL6 Receptor) ; cd-126 IL6R; CD126; IL-6R-1; IL-6R-alpha; IL6RA; MGC104991; IL6 Receptor; CD126 active protein
Host
HEk293 Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Purity/Purification
>97%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Human Interleukin-6 receptor (CD126) is supplied as a 0.2 um filtered PBS solution, pH7.2.
Sequence Length
468
Host Note
Optimized DNA sequence encoding extracellular domain of human IL-6 receptor (CD126) including a C-terminal 6His tag was expressed in HEK293 cells.
Molecular Weight Note
Recombinant IL-6 receptor (CD126) is a monomer protein consisting of 357 amino acid residue subunits, due to glycosylation migrates as an approximately 60-65 kDa protein on SDS-PAGE.
Biological Activity
Recombinant IL-6R (CD126) activity was determined by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 20-80 ng/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Preparation and Storage
Recombinant human IL-6R, as supplied, can be stored in working aliquots at 2°- 8°C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Product Categories/Family for CD126 active protein
References
• anti-inflammatory effect of IL-6 receptor blockade on experimental corneal alkali burn .Invest. Ophthalmol. Vis. Sci., Mar 2012; 53: 4206.
• exposure-response relationship of tocilizumab, an anti–IL-6 receptor monoclonal antibody, in a large population of patients with rheumatoid arthritis .J. Clin. Pharmacol., Feb 2012; 10.1177/0091270012437585.
• impact of IL-6 receptor inhibition on human memory b cells in vivo: impaired somatic hypermutation in preswitch memory b cells and modulation of mutational targeting in memory b cells .Ann Rheum Dis, Aug 2011; 70: 1507 - 1510.
• species specificity of adam10 and adam17 proteins in interleukin-6 (il-6) trans-signaling and novel role of adam10 in inducible IL-6 receptor shedding .J. Biol. Chem., Apr 2011; 286: 14804 - 14811.
• anti-IL-6 receptor antibody (tocilizumab) : a b cell targeting therapy .Ann Rheum Dis, Mar 2011; 70: A57 - A58.
• exposure-response relationship of tocilizumab, an anti–IL-6 receptor monoclonal antibody, in a large population of patients with rheumatoid arthritis .J. Clin. Pharmacol., Feb 2012; 10.1177/0091270012437585.
• impact of IL-6 receptor inhibition on human memory b cells in vivo: impaired somatic hypermutation in preswitch memory b cells and modulation of mutational targeting in memory b cells .Ann Rheum Dis, Aug 2011; 70: 1507 - 1510.
• species specificity of adam10 and adam17 proteins in interleukin-6 (il-6) trans-signaling and novel role of adam10 in inducible IL-6 receptor shedding .J. Biol. Chem., Apr 2011; 286: 14804 - 14811.
• anti-IL-6 receptor antibody (tocilizumab) : a b cell targeting therapy .Ann Rheum Dis, Mar 2011; 70: A57 - A58.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin-6 receptor subunit alpha isoform 1
UniProt Protein Name
Interleukin-6 receptor subunit alpha
UniProt Gene Name
IL6R
UniProt Synonym Gene Names
IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; gp80
UniProt Entry Name
IL6RA_HUMAN
Similar Products
Product Notes
The CD126 il6r (Catalog #AAA76077) is an Active Protein produced from HEk293 Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IL-6 Receptor (IL-6R), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.