CD32b (FcgR2b) Active Protein | CD32 active protein
Human CD32b Recombinant (FcgR2b)
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
CD32b (FcgR2b); N/A; Human CD32b Recombinant (FcgR2b); Human CD32b Recombinant (Fcg RIIb) ; Human CD32b Recombinant (Fcgamma RIIb) ; cd32; cd-32; fcgr3a; CD32 active protein
Host
HEk293 Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant CD32b is supplied as a lyophilized 0.2 um filtered PBS solution, pH7.2.
Sequence Length
217
Host Note
Optimized DNA sequence encoding Human extracellular domain of human CD32b including a C-terminal 6His tag was expressed in HEK293 cells.
Biological Activity
The activity was determined by the ability to bind human IgG2 with a linear range of 0.1-6 ug/ml
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.
Molecular Weight Note
Recombinant CD32b is a monomer protein consisting of 183 amino acid residue subunits, due to glycosylation migrates as an approximately 30 kDa protein on SDS-PAGE.
Preparation and Storage
Recombinant CD32b, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Related Product Information for CD32 active protein
Human CD32B (FcγRIIB), the low-affinity inhibitory Fcγ receptor (FcγR), is highly homologous in its extracellular domain to CD32A (FcγRIIA), an activating FcγR.CD32B is coexpressed with activating Fc receptors on monocytes, macrophages, basophils and mast cells. CD32B is also expressed by B lymphocytes, the sole FcγR expressed by these cells, where it functions by countering B- cell receptor (BCR) -induced activation.
Product Categories/Family for CD32 active protein
References
• CD32b expression reflects phenotypic and functional heterogeneity in multiple myeloma (mm) .Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 842.
• CD32b is highly expressed on clonal plasma cells from patients with systemic light-chain amyloidosis and provides a target for monoclonal antibody–based therapy .Blood, Apr 2008; 111: 3403 - 3406.
• an fc-optimized monoclonal antibody against CD32b, the human inhibitory fc- receptor iib, in the immunotherapy of b-cell malignanacy. .Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 2529.
• CD32b on clonal plasma cells in systemic light-chain amyloidosis (al) and multiple myeloma (mm) : a target for immunotherapy in both disorders and a prognostic marker in mm. .Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 3489.
• CD32b is highly expressed on clonal plasma cells from patients with systemic light-chain amyloidosis and provides a target for monoclonal antibody–based therapy .Blood, Apr 2008; 111: 3403 - 3406.
• an fc-optimized monoclonal antibody against CD32b, the human inhibitory fc- receptor iib, in the immunotherapy of b-cell malignanacy. .Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 2529.
• CD32b on clonal plasma cells in systemic light-chain amyloidosis (al) and multiple myeloma (mm) : a target for immunotherapy in both disorders and a prognostic marker in mm. .Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 3489.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor II-b isoform 2
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II-b
UniProt Gene Name
FCGR2B
UniProt Synonym Gene Names
CD32; FCG2; IGFR2; IgG Fc receptor II-b; Fc-gamma-RIIb; FcRII-b
UniProt Entry Name
FCG2B_HUMAN
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Product Notes
The CD32 fcgr2b (Catalog #AAA76086) is an Active Protein produced from HEk293 Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD32b (FcgR2b), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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