ICOS ligand Active Protein | ICOSLG active protein

ICOS ligand Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA258141

• Gene Names: Icosl; B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653
• Purity: >95% as determined by SDS-PAGE
• Synonyms: ICOS ligand; N/A; ICOS ligand Protein, Mouse, Recombinant (His Tag); Mouse ICOS Ligand/B7-H2/ICOSLG Protein (His Tag); B7-H2 Protein, Mouse; B7h Protein, Mouse; B7RP-1 Protein, Mouse; GI50 Protein, Mouse; GL50 Protein, Mouse; GL50-B Protein, Mouse; ICOS-L Protein, Mouse; Icoslg Protein, Mouse; LICOS Protein, Mouse; inducible T-cell co-stimulator ligand; ICOSLG active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys279

• Species: Mouse
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse B7-H2 at 1ug/ml (100uL/well) can bind human ICOS with a linear range of 40-1000ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 47
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse B7-H2 (NP_056605.1) extracellular domain (Met 1-Lys 279) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized mouse B7-H2 at 1 ug/ml (100 ul/well) can bind human ICOS with a linear range of 40-1000 ng/ml.)

SDS-PAGE

Related Product Information for ICOSLG active protein

Background: Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. The present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production, and germinal center formation.

Product Categories/Family for ICOSLG active protein

Cancer Drug Targets

References

Flesch IE. (2002) Inducible costimulator-ligand (ICOS-L). J Biol Regul Homeost Agents. 16(3): 217-9.Kajiwara K, et al. (2009) Expression and function of the inducible costimulator ligand B7-H2 in human airway smooth muscle cells. Allergol Int. 58(4): 573-83.Wong SC, et al. (2009) Functional hierarchy and relative contribution of the CD28/B7 and ICOS/B7-H2 costimulatory pathways to T cell-mediated delayed-type hypersensitivity. Cell Immunol. 256(1-2): 64-71.

NCBI and Uniprot Product Information

• NCBI GI #: 7657220
• NCBI GeneID: 50723
• NCBI Accession #: NP_056605.1
• NCBI GenBank Nucleotide #: NM_015790.3
• UniProt Accession #: Q9JHJ8
• Molecular Weight: 38,914 Da
• NCBI Official Full Name: ICOS ligand
• NCBI Official Synonym Full Names: icos ligand
• NCBI Official Symbol: Icosl
• NCBI Official Synonym Symbols: B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653
• NCBI Protein Information: ICOS ligand
• UniProt Protein Name: ICOS ligand
• UniProt Gene Name: Icoslg
• UniProt Synonym Gene Names: B7h2; B7rp1; Icosl; B7-H2; B7RP-1
• UniProt Entry Name: ICOSL_MOUSE