Leptin Receptor Active Protein | LEPR active protein

Leptin Receptor Protein, Human, Recombinant (His & hFc Tag)

Cat. Num. AAA257869

• Gene Names: LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Leptin Receptor; N/A; Leptin Receptor Protein, Human, Recombinant (His & hFc Tag); Human Leptin Receptor/LEPR/CD295 Protein (His & Fc Tag); CD295 Protein, Human; LEP-R Protein, Human; LEPRD Protein, Human; OB-R Protein, Human; OBR Protein, Human; leptin receptor; LEPR active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp839

• Species: Human
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 1.25ug/ml (100uL/well) can bind human Leptin receptor with a linear range of 0.032-4.0ug/ml.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Phe 22
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Asp 839) of human leptin receptor precursor (NP_002294.2) was expressed with the C-terminal fused polyhistidine-tagged Fc region of human IgG1.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 1.25 ug/ml (100 ul/well) can bind human Leptin receptor with a linear range of 0.032-4 ug/ml.)

SDS-PAGE

Related Product Information for LEPR active protein

Background: Leptin Receptor or CD295 belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight) and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Leptin Receptor/CD295 is transmembrane catalytic receptors found on NPY/AgRP and alpha-MSH/CART neurons in hypothalamic nuclei. Leptin receptors (Ob-Rs) are coded for by one human gene that produces six different isoforms; Ob-Ra - Ob-Rf. Ob-Rs exist as constitutive dimers at physiological expression levels. Only the Ob-Rb isoform can transduce intracellular signals and does so through activation of the JAK2/STAT3, PI 3-K, and MAPK signaling cascades. Activation of Ob-Rs mediates transcriptional regulation of the hypothalamic melanocortin pathway and downregulates endocannabinoid expression. Leptin acts via leptin receptors. Leptin resistance has been proposed as a pathophysiological mechanism of obesity. In obese individuals, Ob-Ra (which is involved in the active transport of leptin across the blood-brain barrier) expression is downregulated and the individual may be unresponsive to leptin signals. Ob-R antagonists are of great interest in the development of pharmacological treatments for obesity. Mutations in the Leptin Receptor/CD295 have been associated with obesity and pituitary dysfunction.

References

Heshka JT, et al. (2001) A role for dietary fat in leptin receptor, OB-Rb, function. Life Sci. 69 (9): 987-1003.Chen H, et al (1996) Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice. Cell. 84 (3): 491-5.Bjrbaek C, et al. (1998) Divergent signaling capacities of the long and short isoforms of the leptin receptor. J Biol Chem. 272 (51): 32686-95.

NCBI and Uniprot Product Information

• NCBI GI #: 40254464
• NCBI GeneID: 3953
• NCBI Accession #: NP_002294.2
• NCBI GenBank Nucleotide #: NM_002303.5
• UniProt Accession #: P48357; Q13592; Q13593; Q13594; Q92919; Q92920; Q92921
• Molecular Weight: 96,042 Da
• NCBI Official Full Name: leptin receptor isoform 1
• NCBI Official Synonym Full Names: leptin receptor
• NCBI Official Symbol: LEPR
• NCBI Official Synonym Symbols: OBR; OB-R; CD295; LEP-R; LEPRD
• NCBI Protein Information: leptin receptor; huB219; OB receptor
• UniProt Protein Name: Leptin receptor
• UniProt Gene Name: LEPR
• UniProt Synonym Gene Names: DB; OBR; LEP-R; OB-R
• UniProt Entry Name: LEPR_HUMAN