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Application Data
(Loaded Anti-CD112 Antibody on AMC Biosensor, can bind Nectin-2 Protein, Human, Recombinant (His Tag) with an affinity constant of 34.4 nM as determined by Octet RED System.)
Nectin-2 Active Protein | NECTIN2 active protein
Nectin-2 Protein, Human, Recombinant (ECD, His Tag)
Gene Names
NECTIN2; HVEB; PRR2; CD112; PVRL2; PVRR2
Purity
>95% as determined by SDS-PAGE
Synonyms
Nectin-2; N/A; Nectin-2 Protein, Human, Recombinant (ECD, His Tag); Human CD112/Nectin-2/PVRL2 Protein (ECD, His Tag); CD112 Protein, Human; HVEB Protein, Human; Nectin-2 Protein, Human; PRR2 Protein, Human; PVRR2 Protein, Human; poliovirus receptor-related 2 (herpesvirus entry mediator B); NECTIN2 active protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Leu360
Species
Human
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Gln 32
Tag
C-His
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Leu 360) of human CD112 (NP_002847.1) was expressed with a C-terminal polyhistidine tag.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD112 at 20ug/ml (100uL/well) can bind biotinylated DNAM1 with a linear range of 0.078-2.5ug/ml.
2. Using the Octet RED System. The affinity constant (Kd) of Anti-CD112 Antibody bound to Nectin-2 Protein, Human, Recombinant (His Tag) was 34.4nM.
2. Using the Octet RED System. The affinity constant (Kd) of Anti-CD112 Antibody bound to Nectin-2 Protein, Human, Recombinant (His Tag) was 34.4nM.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Application Data
(Loaded Anti-CD112 Antibody on AMC Biosensor, can bind Nectin-2 Protein, Human, Recombinant (His Tag) with an affinity constant of 34.4 nM as determined by Octet RED System.)
Application Data
(Loaded Anti-CD112 Antibody on AMC Biosensor, can bind Nectin-2 Protein, Human, Recombinant (His Tag) with an affinity constant of 34.4 nM as determined by Octet RED System.)
Application Data
(Using the Octet RED System, the affinity constant (Kd) of Nectin-2 Protein, Human, Recombinant (His Tag) bound Anti-CD112 Antibody was 0.1uM.)
Application Data
(Using the Octet RED System, the affinity constant (Kd) of Nectin-2 Protein, Human, Recombinant (His Tag) bound Anti-CD112 Antibody was 0.1uM.)
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD112 at 20 ug/ml (100 ul/well) can bind biotinylated DNAM1 with a linear range of 0.078-2.5 ug/ml.)
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD112 at 20 ug/ml (100 ul/well) can bind biotinylated DNAM1 with a linear range of 0.078-2.5 ug/ml.)
SDS-PAGE
SDS-PAGE
Related Product Information for NECTIN2 active protein
Background: Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
References
Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,359 Da
NCBI Official Full Name
nectin-2 isoform alpha
NCBI Official Synonym Full Names
nectin cell adhesion molecule 2
NCBI Official Symbol
NECTIN2
NCBI Official Synonym Symbols
HVEB; PRR2; CD112; PVRL2; PVRR2
NCBI Protein Information
nectin-2
UniProt Protein Name
Nectin-2
UniProt Gene Name
NECTIN2
UniProt Synonym Gene Names
Herpesvirus entry mediator B; HveB
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Product Notes
The NECTIN2 nectin2 (Catalog #AAA257812) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Leu36 0. It is sometimes possible for the material contained within the vial of "Nectin-2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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