ELISA
(Biotinylated TGFB-1 ELISAImmobilized Biotinylated Human TGF Beta1 at 1ug/ml (100ul/Well). Response curve for TGFR2 with alinear range of 2-100 ng/ml was determined by ELISA)
TGF Beta-1 Active Protein | TGFBeta-1 active protein
Biotinylated Human TGF Beta-1 Recombinant
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Purity
>90%, as determined by SDS-PAGE and HPLC
Synonyms
TGF Beta-1; N/A; Biotinylated Human TGF Beta-1 Recombinant; TGFBeta-1 active protein
Host
HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>90%, as determined by SDS-PAGE and HPLC
Form/Format
Biotinylated Recombinant TGF-b1 is lyophilized from 0.2 um filtered PBS solution pH 7.4.
Source
DNA sequence encoding Human Transforming Growth Factor Beta-1 mature chain was expressed in HEK293 cells fused to C terminal AVI tag
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Biological Activity
The ED(50) was determined by in a functional ELISA assay binding human TGFBR2 at 1ug/ml and was determined to be 15ng/ml.
Biotinylation
TGFB1 is biotinylated in-vitro at a single site using the AVItag biotinylation technology.
Molecular Function
Growth-factor, Mitogen
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months. Avoid repeated freeze/thaw cycles.
ELISA
(Biotinylated TGFB-1 ELISAImmobilized Biotinylated Human TGF Beta1 at 1ug/ml (100ul/Well). Response curve for TGFR2 with alinear range of 2-100 ng/ml was determined by ELISA)
ELISA
(Biotinylated TGFB-1 ELISAImmobilized Biotinylated Human TGF Beta1 at 1ug/ml (100ul/Well). Response curve for TGFR2 with alinear range of 2-100 ng/ml was determined by ELISA)
Tris-Bis-PAGE
(Biotinylated TGFB-1 protein PAGE1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.)
Tris-Bis-PAGE
(Biotinylated TGFB-1 protein PAGE1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.)
Product Categories/Family for TGFBeta-1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,341 Da
NCBI Official Full Name
transforming growth factor beta-1
NCBI Official Synonym Full Names
transforming growth factor, beta 1
NCBI Official Symbol
TGFB1
NCBI Official Synonym Symbols
CED; LAP; DPD1; TGFB; TGFbeta
NCBI Protein Information
transforming growth factor beta-1; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide
UniProt Protein Name
Transforming growth factor beta-1
UniProt Gene Name
TGFB1
UniProt Synonym Gene Names
TGFB; TGF-beta-1; LAP
UniProt Entry Name
TGFB1_HUMAN
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Product Notes
The TGFBeta-1 tgfb1 (Catalog #AAA76386) is an Active Protein produced from HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TGF Beta-1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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