Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse CD105 at 10 ug/ml (100 ul/well) can bind human TGFRB1 with a linear ranger of 6.4-800 ng/ml.)
alk5 Active Protein | TGFBR1 active protein
alk5 Protein, Human, Recombinant (His & hFc Tag)
Gene Names
TGFBR1; AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4
Purity
>85% as determined by SDS-PAGE
Synonyms
alk5; N/A; alk5 Protein, Human, Recombinant (His & hFc Tag); Human TGFBR1/ALK-5/SKR4 Protein (His & Fc Tag); AAT5 Protein, Human; ACVRLK4 Protein, Human; ALK-5 Protein, Human; ALK5 Protein, Human; ESS1 Protein, Human; LDS1 Protein, Human; LDS1A Protein, Human; LDS2A Protein, Human; MSSE Protein, Human; SKR4 Protein, Human; tbetaR-I Protein, Human; TGFR-1 Protein, Human; transforming growth factor, beta receptor 1; TGFBR1 active protein
Host
HEK293 Cells
Purity/Purification
>85% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Glu125
Species
Human
Activity
1. Measured by its binding ability in a functional ELISA. Immobilized mouse CD105 at 10ug/ml (100uL/well) can bind human TGFRB1 with a linear range of 6.4-800ng/mL.
2. Measured by its ability to bind human CD105 in a functional ELISA.
2. Measured by its ability to bind human CD105 in a functional ELISA.
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Leu 34
Tag
C-human IgG1-Fc & His
Protein Construction
A DNA sequence encoding the human TGFBR1 (NP_004603.1) extracellular domain (Met 1-Glu 125) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse CD105 at 10 ug/ml (100 ul/well) can bind human TGFRB1 with a linear ranger of 6.4-800 ng/ml.)
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse CD105 at 10 ug/ml (100 ul/well) can bind human TGFRB1 with a linear ranger of 6.4-800 ng/ml.)
SDS-PAGE
SDS-PAGE
Related Product Information for TGFBR1 active protein
Background: Transforming growth factor, beta receptor I, also known as Transforming growth factor-beta receptor type I, Serine/threonine-protein kinase receptor R4, Activin receptor-like kinase 5, SKR4, ALK-5, and TGFBR1, is a single-pass type I membrane protein that belongs to the protein kinase superfamily and TGFB receptor subfamily. TGFBR1/ALK-5 is found in all tissues examined. It is most abundant in placenta and least abundant in brain and heart. TGF-beta functions as a tumor suppressor by inhibiting the cell cycle in the G1 phase. Administration of TGF-beta is able to protect against mammary tumor development in transgenic mouse models in vivo. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers, with the majority of colon and gastric cancers being caused by an inactivating mutation of TGF-beta RII. On ligand binding, TGFBR1/ALK-5 forms a receptor complex consisting of two type I I and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which auto-phosphorylate, then bind and activate SMAD transcriptional regulators. TGF-beta signaling via TGFBR1/ALK-5 is not required in myocardial cells during mammalian cardiac development, but plays an irreplaceable cell-autonomous role regulating cellular communication, differentiation and proliferation in endocardial and epicardial cells. Defects in TGFBR1/ALK-5 are the cause of Loeys-Dietz syndrome type 1A (LDS1A), Loeys-Dietz syndrome type 2A (LDS2A), and aortic aneurysm familial thoracic type 5 (AAT5).
References
Seki T, et al. (2006) Nonoverlapping expression patterns of ALK1 and ALK5 reveal distinct roles of each receptor in vascular development. Lab Invest. 86(2): 116-29. et al.Piek E, et al. (1999) TGF-(beta) type I receptor/ALK-5 and Smad proteins mediate epithelial to mesenchymal transdifferentiation in NMuMG breast epithelial cells. J Cell Sci. 112 (24): 4557-68. et al.Dudas M, et al. (2004) Tgf-beta3-induced palatal fusion is mediated by Alk-5/Smad pathway. Dev Biol. 266(1): 96-108.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,960 Da
NCBI Official Full Name
TGF-beta receptor type-1 isoform 1
NCBI Official Synonym Full Names
transforming growth factor, beta receptor 1
NCBI Official Symbol
TGFBR1
NCBI Official Synonym Symbols
AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4
NCBI Protein Information
TGF-beta receptor type-1; tbetaR-I; TGF-beta receptor type I; TGF-beta type I receptor; activin receptor-like kinase 5; multiple self-healing squamous epithelioma; transforming growth factor beta receptor I; serine/threonine-protein kinase receptor R4; ac
UniProt Protein Name
TGF-beta receptor type-1
UniProt Gene Name
TGFBR1
UniProt Synonym Gene Names
ALK5; SKR4; TGFR-1; ALK-5; ALK5; SKR4; TGF-beta receptor type I; TbetaR-I
UniProt Entry Name
TGFR1_HUMAN
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Product Notes
The TGFBR1 tgfbr1 (Catalog #AAA257886) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Glu12 5. It is sometimes possible for the material contained within the vial of "alk5, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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