IP (Immunoprecipitation)
(ALPL was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 4 uL anti- ALPL rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- ALPL rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57kDa Observed band size :80 kDa)
Rabbit anti-Human Alkaline Phosphatase/ALPL Polyclonal Antibody | anti-TGFBR1 antibody
Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Polyclonal
Gene Names
TGFBR1; AAT5; ALK5; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4
Reactivity
Human
Applications
ELISA
Purity
Protein A & Antigen Affinity
Synonyms
Alkaline Phosphatase/ALPL, Antibody; Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Polyclonal; alk5/TGFBR1 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-AP-TNAP Antibody; Anti-APTNAP Antibody; Anti-HOPS Antibody; Anti-TNAP Antibody; Anti-TNSALP Antibody; alkaline phosphatase, liver/bone/kidney; anti-TGFBR1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human alk5/TGFBR1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TGFBR1 antibody
ELISA
Conjugation
Unconjugated
Immunogen
Recombinant Human Alkaline Phosphatase/ALPL Protein;
Preparation
Produced in rabbits immunized with purified, recombinant Human alk5/TGFBR1 (rh alk5/TGFBR1;P36897-1; Thr200-Met503). alk5/TGFBR1 specific IgG was purified by Human alk5/TGFBR1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(ALPL was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 4 uL anti- ALPL rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- ALPL rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57kDa Observed band size :80 kDa)
IP (Immunoprecipitation)
(ALPL was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 4 uL anti- ALPL rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- ALPL rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57kDa Observed band size :80 kDa)
WB (Western Blot)
(Anti-Alkaline Phosphatase/ALPL rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:57 kDa)
WB (Western Blot)
(Anti-Alkaline Phosphatase/ALPL rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:57 kDa)
Related Product Information for anti-TGFBR1 antibody
Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.
Product Categories/Family for anti-TGFBR1 antibody
References
Brun-Heath I, et al. (2011) Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues. Cell Tissue Res. 343(3): 521-36.Whyte MP, et al. (1995) Alkaline phosphatase: placental and tissue-non-specific isoenzymes hydrolyze phosphoethanolamine, inorganic pyrophosphate, and pyridoxal 5-phosphate. J Clin Invest. 95: 1440-5.Whyte MP. (1994) Hypophosphatasia and the role of alkaline phosphatase in skeletal mineralization. Endocrinol Rev. 4: 439-61.Weinreb M, et al. (1990) Different pattern of alkaline phosphatase, osteopontin and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Miner Res. 5: 831-42.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,960 Da
NCBI Official Full Name
TGF-beta receptor type-1 isoform 2
NCBI Official Synonym Full Names
transforming growth factor, beta receptor 1
NCBI Official Symbol
TGFBR1
NCBI Official Synonym Symbols
AAT5; ALK5; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4
NCBI Protein Information
TGF-beta receptor type-1; tbetaR-I; TGF-beta receptor type I; TGF-beta type I receptor; activin receptor-like kinase 5; transforming growth factor beta receptor I; serine/threonine-protein kinase receptor R4; activin A receptor type II-like kinase, 53kD;
UniProt Protein Name
TGF-beta receptor type-1
UniProt Gene Name
TGFBR1
UniProt Synonym Gene Names
ALK5; SKR4; TGFR-1; ALK-5; ALK5; SKR4; TGF-beta receptor type I; TbetaR-I
UniProt Entry Name
TGFR1_HUMAN
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Product Notes
The TGFBR1 tgfbr1 (Catalog #AAA258515) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alkaline Phosphatase/ALPL can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the TGFBR1 tgfbr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase/ALPL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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