CD4 protein

Biotinylated Human CD4 full length protein-synthetic nanodisc

Cat. Num. AAA129477

• Gene Names: CD4; CD4mut
• Synonyms: CD4; N/A; Biotinylated Human CD4 full length protein-synthetic nanodisc; T4; IMD79; Leu-3; OKT4D; CD4mut; CD4 protein

• Host: HEK293
• Form/Format: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5%-8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.

• Tag: C-DYKDDDDK&Avi
• Conjugation: Biotinylated
• Preparation and Storage: Store at -20 degree C to -80 degree C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80 degree C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

SDS-PAGE

(Figure 2. Biotinylated Human CD4-Nanodisc, DYKDDDDK Tag on SDS-PAGE)

ELISA

(Figure 1. ELISA plate pre-coated by 0.2 ug/mL (100 uL/well) Anti-DYKDDDDK monoclonal antibody can bind Biotinylated Human CD4 full length protein-synthetic nanodisc (AAA129477) in a linear range of 16–2000 ng/mL. In order to specifically detect AAA129477, HRP Conjugated Streptavidin was used as detection antibody.)

Related Product Information for CD4 protein

This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, May 2020]

Product Categories/Family for CD4 protein

Antigen processing and presentation; Cell adhesion molecules (CAMs); Hematopoietic cell lineage; Primary immunodeficiency; T cell receptor signaling pathway

NCBI and Uniprot Product Information

• NCBI GI #: 10835167
• NCBI GeneID: 920
• NCBI Accession #: NP_000607.1
• NCBI GenBank Nucleotide #: NM_000616.4
• UniProt Accession #: P01730; Q4ZGK2; Q5U066; Q9UDE5; B2R737; D3DUS5
• Molecular Weight: 51,111 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4 isoform 1
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
• UniProt Protein Name: T-cell surface glycoprotein CD4
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_HUMAN