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product-image-AAA282571_AD11.jpg Application Data (Recombinant Human HGF(20 ng/mL) and KGF(20 ng/mL induce iPSC-derived mesoderm and endoderm cells for 6 days to differentiate into hepatocytes. According to flow cytometry results, the signals of hepatocytes markers AFP and SOX9 turn positive after induction.(Customer feedback data))

Hepatocyte growth factor/HGF Recombinant Protein | HGF recombinant protein

Recombinant Human Hepatocyte growth factor/HGF Protein

Gene Names
HGF; SF; HGFB; HPTA; F-TCF; DFNB39
Purity
> 95% by SDS-PAGE.
Synonyms
Hepatocyte growth factor/HGF; N/A; Recombinant Human Hepatocyte growth factor/HGF Protein; HGF; DFNB39; F-TCF; HGFB; HPTA; SF; HGF recombinant protein
Ordering
Host
HEK293 cells
Purity/Purification
> 95% by SDS-PAGE.
Form/Format
Liquid
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Species
Human
Tag
C-His
Bioactivity
Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is 2.23-8.91 ng/mL, corresponding to a specific activity of 1.12^105-4.48^105 units/mg.
Preparation and Storage
Store the lyophilized protein at -20 to -80 degree C for 12 months. After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week. Avoid repeated freeze/thaw cycles.
Ship: Ice Pack

Application Data

(Recombinant Human HGF(20 ng/mL) and KGF(20 ng/mL induce iPSC-derived mesoderm and endoderm cells for 6 days to differentiate into hepatocytes. According to flow cytometry results, the signals of hepatocytes markers AFP and SOX9 turn positive after induction.(Customer feedback data))

product-image-AAA282571_AD11.jpg Application Data (Recombinant Human HGF(20 ng/mL) and KGF(20 ng/mL induce iPSC-derived mesoderm and endoderm cells for 6 days to differentiate into hepatocytes. According to flow cytometry results, the signals of hepatocytes markers AFP and SOX9 turn positive after induction.(Customer feedback data))

Application Data

(Recombinant Human HGF induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is 2.23-8.91 ng/mL, corresponding to a specific activity of 1.12×105-4.48×105 units/mg.)

product-image-AAA282571_AD13.jpg Application Data (Recombinant Human HGF induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is 2.23-8.91 ng/mL, corresponding to a specific activity of 1.12×105-4.48×105 units/mg.)

SDS-PAGE

(Active Recombinant Human Hepatocyte growth factor/HGF Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 100-110 kDa.)

product-image-AAA282571_SDS_PAGE15.jpg SDS-PAGE (Active Recombinant Human Hepatocyte growth factor/HGF Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 100-110 kDa.)
Related Product Information for HGF recombinant protein
Active Recombinant Human Hepatocyte growth factor/HGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln32-Ser728) of human Hepatocyte growth factor/HGF (Accession #NP_000592.3) fused with and a 6xHis tag at the C-terminus.
Product Categories/Family for HGF recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
728
NCBI Official Full Name
Hepatocyte growth factor
NCBI Official Synonym Full Names
hepatocyte growth factor (hepapoietin A; scatter factor)
NCBI Official Symbol
HGF
NCBI Official Synonym Symbols
SF; HGFB; HPTA; F-TCF; DFNB39
NCBI Protein Information
hepatocyte growth factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor
UniProt Protein Name
Hepatocyte growth factor
UniProt Gene Name
HGF
UniProt Synonym Gene Names
HPTA; SF
UniProt Entry Name
HGF_HUMAN

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Product Notes

The HGF hgf (Catalog #AAA282571) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Hepatocyte growth factor/HGF, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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