Application Data
(Mass Spec analysis for Recombinant Mononucleosomes H3R2/8/17 citrul (EPL) Histone H3R2/8/17 citrul (EPL) protein was analyzed by ESI-TOF mass spectrometry. Expected mass = 15229 Da. Determined mass = 15229 Da.)
Mononucleosomes H3R2/8/17 citrul Recombinant Protein | H3R2/8/17 recombinant protein
Recombinant Mononucleosomes H3R2/8/17 citrul (EPL)
Gene Names
HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
Synonyms
Mononucleosomes H3R2/8/17 citrul; N/A; Recombinant Mononucleosomes H3R2/8/17 citrul (EPL); H3R2/8/17 recombinant protein
Host
E Coli
Form/Format
Recombinant Mononucleosomes H3R2/8/17 citrul (EPL)(20ug protein + 20ug DNA) are supplied at a protein concentration of 0.53mg/ml in 10mM Tris-HCl pH8.0, 1mM EDTA, 2mM DTT, and 20% glycerol.
Protein Species
Human
Notes
Recombinant Mononucleosomes H3R2/8/17 citrul (EPL) are suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Mass Spec analysis for Recombinant Mononucleosomes H3R2/8/17 citrul (EPL) Histone H3R2/8/17 citrul (EPL) protein was analyzed by ESI-TOF mass spectrometry. Expected mass = 15229 Da. Determined mass = 15229 Da.)
Application Data
(Mass Spec analysis for Recombinant Mononucleosomes H3R2/8/17 citrul (EPL) Histone H3R2/8/17 citrul (EPL) protein was analyzed by ESI-TOF mass spectrometry. Expected mass = 15229 Da. Determined mass = 15229 Da.)
Application Data
(Recombinant Mononucleosomes H3R2/8/17 citrul (EPL) DNA gel Mononucleosomes H3R2/8/17 citrul were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Intact mononucleosome H3R2/8/17 citrul Lane 3: 601 DNA. Intact mononucleosome H3R2/8/17 citrul migrated higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wraps histone octamers to form nucleosomes.)
Application Data
(Recombinant Mononucleosomes H3R2/8/17 citrul (EPL) DNA gel Mononucleosomes H3R2/8/17 citrul were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Intact mononucleosome H3R2/8/17 citrul Lane 3: 601 DNA. Intact mononucleosome H3R2/8/17 citrul migrated higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wraps histone octamers to form nucleosomes.)
SDS-PAGE
(Recombinant Mononucleosomes H3R2/8/17 citrul (EPL) SDS PAGE gel 13% SDS-PAGE gel, stained with Coomassie blue. Purity: ? 92%)
SDS-PAGE
(Recombinant Mononucleosomes H3R2/8/17 citrul (EPL) SDS PAGE gel 13% SDS-PAGE gel, stained with Coomassie blue. Purity: ? 92%)
Related Product Information for H3R2/8/17 recombinant protein
Short Description: Recombinant Mononucleosomes H3R2/8/17 citrul (EPL) consist of a 167 bp of 601 DNA and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NP_003503.1), H2B that includes amino acids 1-126 (end)(accession number NP_003509.1), H3.2 that includes amino acids 1-136 (end)(accession number NP_066403.2) with citrullination at arginine 2, arginine 8 and arginine 17, and H4 that includes amino acids 1-103 (end)(accession number NP_003539.1). All of these histones were expressed in E Coli. The molecular weight of histone octamer is ~108 kDa. H3R2/8/17 citrul (Histone H3 citrullyl Arg2/8/17) protein is generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E Coli and purified using FPLC. The purified protein is subsequently ligated to an N-terminal histone tail peptide containing citrulline 2/8/17 via a native peptide bond.
Background: In vivo, histones are wrapped around by DNA in chromatin. Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.
Background: In vivo, histones are wrapped around by DNA in chromatin. Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.
Product Categories/Family for H3R2/8/17 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,105 Da
NCBI Official Full Name
histone H2A type 1-C
NCBI Official Synonym Full Names
histone cluster 1 H2A family member c
NCBI Official Symbol
HIST1H2AC
NCBI Official Synonym Symbols
H2A/l; H2AFL; dJ221C16.4
NCBI Protein Information
histone H2A type 1-C
UniProt Protein Name
Histone H2A type 1-C
UniProt Gene Name
HIST1H2AC
UniProt Synonym Gene Names
H2AFL
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Product Notes
The H3R2/8/17 hist1h2ac (Catalog #AAA60170) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Mononucleosomes H3R2/8/17 citrul, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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