Mouse heme oxygenase (decycling) 1 ELISA Kit | HMOX1 elisa kit
Mouse heme oxygenase 1, HO-1 ELISA Kit
Gene Names
Hmox1; HO1; HO-1; Hmox; Hemox; Hsp32; D8Wsu38e
Reactivity
Mouse
Synonyms
heme oxygenase (decycling) 1; N/A; Mouse heme oxygenase 1, HO-1 ELISA Kit; Mouse heme oxygenase 1; HO-1 ELISA Kit; HO-1; HSP32; bK286B10; heme oxygenase (decyclizing) 1; HMOX1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse HO-1. No significant cross-reactivity or interference between mouse HO-1 and analogues was observed.
Sequence Length
289
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates, cell culture supernates
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
< 7.8 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for HMOX1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HO-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HO-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HO-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HO-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,929 Da
NCBI Official Full Name
heme oxygenase 1
NCBI Official Synonym Full Names
heme oxygenase (decycling) 1
NCBI Official Symbol
Hmox1
NCBI Official Synonym Symbols
HO1; HO-1; Hmox; Hemox; Hsp32; D8Wsu38e
NCBI Protein Information
heme oxygenase 1; P32 protein; hemoxygenase
UniProt Protein Name
Heme oxygenase 1
UniProt Gene Name
Hmox1
UniProt Synonym Gene Names
HO-1
UniProt Entry Name
HMOX1_MOUSE