Horse PAI1 (Plasminogen activator inhibitor 1) ELISA Kit | PAI1 elisa kit

Horse PAI1 (Plasminogen activator inhibitor 1)

Cat. Num. AAA102629

• Gene Names: SERPINE1; PAI; PAI1; PAI-1; PLANH1
• Reactivity: Horse
• Synonyms: PAI1 (Plasminogen activator inhibitor 1); N/A; Horse PAI1 (Plasminogen activator inhibitor 1); PAI-1/PAI1/SERPINE1/Nexin/PLANH1/Endothelial plasminogen activator inhibitor/PAISerpin E1/PLANH1/plasminogen activator inhibitor 1/serine (or cysteine) proteinase inhibitor; clade E (nexin; plasminogenactivator inhibitor type 1); member 1/serpin peptidase inhibitor; plasminogen activator inhibitortype 1); member 1; PAI1 elisa kit

• Reactivity: Horse
• Specificity: This assay has high sensitivity and excellent specificity for detection of PAI1 . No significant cross-reactivity or interference between PAI1 and analogues was observed.
• Sequence Length: 402
• Assay Type: Competitive ELISA, Coated with Antigen
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PAI1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PAI1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for PAI1 elisa kit

Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with PAI1 . During the reaction,PAI1 in the sample or standard competes with a fixed amount of PAI1 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to PAI1 . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PAI1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10835159
• NCBI GeneID: 5054
• NCBI Accession #: NP_000593.1
• NCBI GenBank Nucleotide #: NM_000602.4
• Molecular Weight: 43,404 Da
• NCBI Official Full Name: plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serpin family E member 1
• NCBI Official Symbol: SERPINE1
• NCBI Official Synonym Symbols: PAI; PAI1; PAI-1; PLANH1
• NCBI Protein Information: plasminogen activator inhibitor 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• UniProt Gene Name: SERPINE1
• UniProt Synonym Gene Names: PAI1; PLANH1; PAI; PAI-1
• UniProt Entry Name: PAI1_HUMAN