Horse PAI2 (Plasminogen Activator Inhibitor 2) ELISA Kit | PAI2 elisa kit

Horse PAI2 (Plasminogen Activator Inhibitor 2)

Cat. Num. AAA102631

• Gene Names: SERPINB2; PAI; PAI2; PAI-2; PLANH2; HsT1201
• Reactivity: Horse
• Synonyms: PAI2 (Plasminogen Activator Inhibitor 2); N/A; Horse PAI2 (Plasminogen Activator Inhibitor 2); PAI2/PAI2/PLANH2/Urokinase Inhibitor/HsT1201/Monocyte Arg-serpin/PAI/PAI2Urokinase inhibitor/Placental plasminogen activator inhibitor/PLANH2PAI-2/plasminogen activator inhibitor 2/plasminogen activator inhibitor; type II (arginine-serpin)/serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 2/Serpin B2/serpin peptidase inhibitor; member 2; PAI2 elisa kit

• Reactivity: Horse
• Specificity: This assay has high sensitivity and excellent specificity for detection of PAI2 . No significant cross-reactivity or interference between PAI2 and analogues was observed.
• Sequence Length: 415
• Assay Type: Competitive ELISA, Coated with Antigen
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PAI2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PAI2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for PAI2 elisa kit

Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with PAI2 . During the reaction,PAI2 in the sample or standard competes with a fixed amount of PAI2 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to PAI2 . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PAI2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 219689110
• NCBI GeneID: 5055
• NCBI Accession #: NP_001137290.1
• NCBI GenBank Nucleotide #: NM_001143818.1
• Molecular Weight: 46,596 Da
• NCBI Official Full Name: plasminogen activator inhibitor 2
• NCBI Official Synonym Full Names: serpin family B member 2
• NCBI Official Symbol: SERPINB2
• NCBI Official Synonym Symbols: PAI; PAI2; PAI-2; PLANH2; HsT1201
• NCBI Protein Information: plasminogen activator inhibitor 2
• UniProt Protein Name: Plasminogen activator inhibitor 2
• UniProt Gene Name: SERPINB2
• UniProt Synonym Gene Names: PAI2; PLANH2; PAI-2
• UniProt Entry Name: PAI2_HUMAN