Standard Curve (Sample)
(Human Hemopexin PicoKine ELISA Kit standard curve)
Human Hemopexin ELISA Kit | HPX elisa kit
Human Hemopexin PicoKine ELISA Kit
Gene Names
HPX; HX
Reactivity
Human
Synonyms
Hemopexin; N/A; Human Hemopexin PicoKine ELISA Kit; Beta 1B glycoprotein; Beta-1B-glycoprotein; HEMO; Hpx; HX; P02790; HPX elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human Hemopexin
Sequence Length
462
Samples
Cell culture supernates, serum and plasma(heparin, EDTA).
Assay Type
Sandwich
Detection Range
3.12ng/ml-200ng/ml
Sensitivity
<50pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Standard Curve (Sample)
(Human Hemopexin PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human Hemopexin PicoKine ELISA Kit standard curve)
Related Product Information for HPX elisa kit
Principle of the Assay: Human Hemopexin ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for Hemopexin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: T24-H462) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Hemopexin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Hemopexin amount of sample captured in plate.
Background/Introduction: Hemopexin (or haemopexin; HPX), also known as beta-1B-glycoprotein, is a protein that in humans is encoded by the HPX gene and belongs to hemopexin family of proteins. Hemopexin binds heme with the highest affinity of any known protein. Its function is scavenging the heme released or lost by the turnover of heme proteins such as hemoglobin and thus protects the body from the oxidative damage that free heme can cause. In addition, hemopexin releases its bound ligand for internalisation upon interacting with a specific receptor situated on the surface of liver cells. This function of hemopexin is to preserve the body's iron.
Background/Introduction: Hemopexin (or haemopexin; HPX), also known as beta-1B-glycoprotein, is a protein that in humans is encoded by the HPX gene and belongs to hemopexin family of proteins. Hemopexin binds heme with the highest affinity of any known protein. Its function is scavenging the heme released or lost by the turnover of heme proteins such as hemoglobin and thus protects the body from the oxidative damage that free heme can cause. In addition, hemopexin releases its bound ligand for internalisation upon interacting with a specific receptor situated on the surface of liver cells. This function of hemopexin is to preserve the body's iron.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,676 Da
NCBI Official Full Name
hemopexin
NCBI Official Synonym Full Names
hemopexin
NCBI Official Symbol
HPX
NCBI Official Synonym Symbols
HX
NCBI Protein Information
hemopexin
UniProt Protein Name
Hemopexin
UniProt Gene Name
HPX
UniProt Entry Name
HEMO_HUMAN
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Product Notes
The Human HPX hpx (Catalog #AAA46666) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46666 ELISA Kit recognizes Human HPX. It is sometimes possible for the material contained within the vial of "Hemopexin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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