Human C-Reactive Protein ELISA Kit | hsCRP elisa kit

C-Reactive Protein (hsCRP)  ELISA

Cat. Num. AAA14191

• Gene Names: CRP; PTX1
• Reactivity: Human
• Synonyms: C-Reactive Protein; N/A; C-Reactive Protein (hsCRP) ELISA; hsCRP elisa kit

• Reactivity: Human
• Specificity: The specificity of the hs-CRP ELISA kit was determined by measuring the apparent CRP value of samples spiked with the following compounds
• Sequence Length: 224
• Assay Type: Sandwich
• Samples: Human serum
• Sensitivity: 10 ng/ml

• Intra-assay Precision: Three samples were assayed ten times each on the same calibrator curve.
• Inter-assay Precision: Three samples were assayed ten times over a period of four weeks.
• Preparation and Storage: 2 - 8 degree C

Standard Curve (Sample)

Related Product Information for hsCRP elisa kit

Principle of the Assay: The principle of the following enzyme immunoassay test follows a typical two-step capture or 'sandwich' type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for CRP is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of CRP is conjugated to horse radish peroxidase (HRP). CRP from the sample and calibrators are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of CRP in the sample. A set of calibrators is used to plot a standard curve from which the amount of CRP in samples and controls can be directly read.

Intended Uses: For the determination of C-Reactive Protein by enzyme immunoassay in human serum.

Product Categories/Family for hsCRP elisa kit

Cytokines & Cell Signalling; Research Assays; Assay Categories

NCBI and Uniprot Product Information

• NCBI GI #: 55770842
• NCBI GeneID: 1401
• NCBI Accession #: NP_000558.2
• NCBI GenBank Nucleotide #: NM_000567.2
• Molecular Weight: 10,415 Da
• NCBI Official Full Name: C-reactive protein
• NCBI Official Synonym Full Names: C-reactive protein, pentraxin-related
• NCBI Official Symbol: CRP
• NCBI Official Synonym Symbols: PTX1
• NCBI Protein Information: C-reactive protein; pentraxin 1
• UniProt Protein Name: C-reactive protein
• UniProt Gene Name: CRP
• UniProt Synonym Gene Names: PTX1
• UniProt Entry Name: CRP_HUMAN