Heat Shock 70kDa Protein 4 (HSPA4) Recombinant Protein | HSPA4 recombinant protein
Recombinant Heat Shock 70kDa Protein 4 (HSPA4)
Gene Names
HSPA4; RY; APG-2; HSPH2; hsp70; hsp70RY; HS24/P52
Applications
Immunoprecipitation, ELISA, Western Blot, SDS-Page
Purity
> 95%
Synonyms
Heat Shock 70kDa Protein 4 (HSPA4); N/A; Recombinant Heat Shock 70kDa Protein 4 (HSPA4); HSPA4 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDVDVSGT MNRGKFLEMC NDLLARVEPP LRSVLEQTKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGSSDCEVF SKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIAQFSVQKVTPQ SDGSSSKVKV KVRVNVHGIF SVSSASLVEV HKSEENEEPM E
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDVDVSGT MNRGKFLEMC NDLLARVEPP LRSVLEQTKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGSSDCEVF SKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIAQFSVQKVTPQ SDGSSSKVKV KVRVNVHGIF SVSSASLVEV HKSEENEEPM E
Sequence Length
840
Applicable Applications for HSPA4 recombinant protein
IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
Organism
Homo sapiens (Human)
Expression System
Prokaryotic expression
Residues
Ser258~Glu511 (Accession # P34932) with two N-terminal Tags, His-tag and T7-tag
Subcellular Location
Cytoplasm
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
7.1
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31.7kDa
NCBI Official Full Name
heat shock 70 kDa protein 4
NCBI Official Synonym Full Names
heat shock 70kDa protein 4
NCBI Official Symbol
HSPA4
NCBI Official Synonym Symbols
RY; APG-2; HSPH2; hsp70; hsp70RY; HS24/P52
NCBI Protein Information
heat shock 70 kDa protein 4; hsp70 RY; heat shock 70kD protein 4; heat shock protein, 110 kDa; heat shock 70-related protein APG-2
UniProt Protein Name
Heat shock 70 kDa protein 4
UniProt Gene Name
HSPA4
UniProt Synonym Gene Names
APG2
UniProt Entry Name
HSP74_HUMAN
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Product Notes
The HSPA4 hspa4 (Catalog #AAA135483) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Heat Shock 70kDa Protein 4 (HSPA4) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE. Researchers should empirically determine the suitability of the HSPA4 hspa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDVDVSGT MNRGKFLEMC NDLLARVEPP LRSVLEQTKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGSSDCEVF SKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV KVRVNVHGIF SVSSASLVEV HKSEENEEPM E. It is sometimes possible for the material contained within the vial of "Heat Shock 70kDa Protein 4 (HSPA4), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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