SDS-PAGE
(3 ug by SDS-PAGE under reducing condition and visualized by coomasie blue stain)
HSP10 recombinant protein
HSP10, 1-102aa, Human, E Coli
Gene Names
HSPE1; EPF; CPN10; GROES; HSP10
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
HSP10; N/A; HSP10, 1-102aa, Human, E Coli; Heat shock protein 10; Chaperonin 10; CPN10; GroES; Heat shock protein 10 10 kDa chaperonin; HSPE 1; HSPE1; 10 kDa heat shock protein mitochondrial; Cpn 10; Early pregnancy factor; EPF; Heat shock 10kD protein 1; Heat shock 10kD protein 1 chaperonin 10; Heat shock 10kDa protein 1; HSP 10; Heat shock 10kDa protein 1 chaperonin 10,; HSP10 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid in 20mM Tris pH 8.0, 50mM NaCl
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MAGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG SGSKGKGGEI QPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDGDILGKY VD
Sequence Length
102
Applicable Applications for HSP10 recombinant protein
SDS-PAGE
Tag
Non-tagged
Domain
1-102aa
Endotoxin Level
< 1.0 EU per 1 ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at 2-8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3 ug by SDS-PAGE under reducing condition and visualized by coomasie blue stain)
SDS-PAGE
(3 ug by SDS-PAGE under reducing condition and visualized by coomasie blue stain)
Related Product Information for HSP10 recombinant protein
Heat shock protein (HSP) 10 is a member of the highly conserved group of molecular chaperons, which are necessary for efficient folding of many proteins in normal and stress conditions and have been implicated in several human diseases. HSP10 is the ~10kD mammalian equivalent to GroES of E Coli. The function of Hsp10 is to bind Hsp60 (also known as chaperonin-60), in the presence of ATP, thereby promoting a conformational change in Hsp60 and enclosing the protein substrate within the complex. ATP hydrolysis by Hsp60 destabilizes the Hsp60/ Hsp10 complex, allowing it to dissociate and release the substrate protein. Recombinant human HSP10 was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for HSP10 recombinant protein
References
Lin KM., et al. (2004), FASEB J. 18 (9):1004-6; Hohfeld J., et al. (1994), J. Cell BIol. 126 (2):305-15
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10.9 kDa (102 aa) confirmed by MALDI-TOF.
NCBI Official Full Name
10 kDa heat shock protein, mitochondrial
NCBI Official Synonym Full Names
heat shock protein family E (Hsp10) member 1
NCBI Official Symbol
HSPE1
NCBI Official Synonym Symbols
EPF; CPN10; GROES; HSP10
NCBI Protein Information
10 kDa heat shock protein, mitochondrial
UniProt Protein Name
10 kDa heat shock protein, mitochondrial
UniProt Gene Name
HSPE1
UniProt Synonym Gene Names
Hsp10; CPN10; EPF
Similar Products
Product Notes
The HSP10 hspe1 (Catalog #AAA11744) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HSP10 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the HSP10 hspe1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG SGSKGKGGEI QPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDGDILGKY VD. It is sometimes possible for the material contained within the vial of "HSP10, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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