SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Alphaenolase Active Protein
Alphaenolase protein
Applications
SDS-Page
Purity
> 90% pure
Synonyms
Alphaenolase; N/A; Alphaenolase protein; Enolase 1 protein; C myc promoter binding protein protein; 2 phospho D glycerate hydro lyase protein; Alpha enolase protein; Phosphopyruvate hydratase protein; MPB 1 protein; ENO1L1 protein; MYC promoter-binding protein 1 protein; Non neural enolase protein; Enolase 1 (alpha) like 1 protein; Non-Neuronal Enolase (NNE) protein; MYC promoter binding protein 1 protein; EC 4.2.1.11 protein; NNE protein; MBP-1 protein; PPH protein; Enolase-1 protein; MBPB1 protein; Plasminogen binding protein protein; MBP 1 protein; ENO1 protein; Enolase 1 (alpha) protein; tau-crystallin protein; MPB1 protein; MBP1 protein; Alphaenolase active protein
Host
Human
Purity/Purification
> 90% pure
Form/Format
Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1 mM MgSO4 and 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence
1-434 amino acids: MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK
Sequence Length
434
Applicable Applications for Alphaenolase active protein
SDS-PAGE
Protein Type
Recombinant
Biological Significance
Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoprotein associated with Hashimoto's encephalopathy and severe asthma. Recombinant human alpha-enolase was expressed in E Coli and purified by using conventional chromatography.
Bioactivity
Specific activity: > 13 units/ml. One unit will convert 1.0 umole of 2-phosphoglycerate to phospho(enol)pyruvate per minute at pH 7.5 at 25 degree C
Expression System
E Coli
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for Alphaenolase active protein
Purified recombinant Human Alphaenolase protein
Product Categories/Family for Alphaenolase active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
47.1 kDa (434aa), confirmed by MALDI-TOF.
NCBI Official Full Name
Alpha-enolase
Similar Products
Product Notes
The Alphaenolase (Catalog #AAA75217) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Alphaenolase can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Alphaenolase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 1-434 amino acids: MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK. It is sometimes possible for the material contained within the vial of "Alphaenolase, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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