FCM/FACS (Flow Cytometry)
(Separation of human thrombocytes (red-filled) from CD151 negative neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD151 (50-6) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
anti-Human CD151 Antibody | anti-CD151 antibody
Anti-Hu CD151 APC
Gene Names
CD151; GP27; MER2; RAPH; SFA1; PETA-3; TSPAN24
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD151; N/A; Anti-Hu CD151 APC; CD151 molecule (Raph blood group); RAPH blood group; MER2; SFA1; PETA-3; GP27; anti-CD151 antibody
Reactivity
Human
Isotype
Mouse IgG1 kappa
Clone Number
50-6
Specificity
The mouse monoclonal antibody CD151 recognizes an extracellular epitope of CD151 (also known as PETA-3), a 29 kDa transmembrane protein of tetraspanin family, expressed in many cell types.
Form/Format
APC
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m
Applicable Applications for anti-CD151 antibody
FCM/FACS (Flow Cytometry)
Immunogen
Human epidermoid carcinoma cell line Hep-3
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human thrombocytes (red-filled) from CD151 negative neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD151 (50-6) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human thrombocytes (red-filled) from CD151 negative neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD151 (50-6) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD151 (50-6) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD151 (50-6) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
Related Product Information for anti-CD151 antibody
CD151, also known as PETA-3 (platelet-endothelial tetraspan antigen), is a four-pass transmembrane glycoprotein with short cytoplasmic N- and C-termini. CD151 is expressed mainly in platelets and megakaryocytes, immature hematopoietic cells, activated T cells, in endothelium, muscle cells, and epithelial cells. It associates with CD9, CD181, and integrin complexes alpha 3/beta 1 (CD49c/CD29), alpha 5/beta 1 (CD49e/CD29), and alpha 6/beta 4 (CD49f/CD104). CD151 appears to be involved in cell adhesion and migration, including metastasis.
References
*Sadej R, Grudowska A, Turczyk L, Kordek R, Romanska HM: CD151 in cancer progression and metastasis: a complex scenario. Lab Invest. 2014 Jan;94(1):41-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/24247563,*Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, Anstee DJ: Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1. PLoS One. 2013 May 21;8(5):e62654., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=spring+fa+griffiths+re Testa JE Brooks PC Lin JM Quigley JP: Eukaryotic expression cloning with an antimetastatic monoclonal antibody identifies a tetraspanin (PETA-3/CD151) as an effector of human tumor cell,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,295 Da
NCBI Official Full Name
CD151 antigen
NCBI Official Synonym Full Names
CD151 molecule (Raph blood group)
NCBI Official Symbol
CD151
NCBI Official Synonym Symbols
GP27; MER2; RAPH; SFA1; PETA-3; TSPAN24
NCBI Protein Information
CD151 antigen; tspan-24; tetraspanin-24; membrane glycoprotein SFA-1; CD151 antigen (Raph blood group); hemidesmosomal tetraspanin CD151; platelet surface glycoprotein gp27; platelet-endothelial tetraspan antigen 3; platelet-endothelial cell tetraspan ant
UniProt Protein Name
CD151 antigen
UniProt Gene Name
CD151
UniProt Synonym Gene Names
TSPAN24; PETA-3; Tspan-24
UniProt Entry Name
CD151_HUMAN
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Product Notes
The CD151 cd151 (Catalog #AAA129302) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD151 APC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD151 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD151 cd151 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD151, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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