FCM/FACS (Flow Cytometry)
(Separation of human CD158agh positive CD3 negative lymphocytes (red-filled) from CD158agh negative CD3 positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD158agh (HP-MA4) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
anti-Human CD158agh Antibody | anti-CD158agh antibody
Anti-Hu CD158agh PE-Cy7
Gene Names
KIR2DL4; G9P; CD158D; KIR103; KIR-2DL4; KIR103AS; KIR-103AS
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD158agh; N/A; Anti-Hu CD158agh PE-Cy7; killer cell immunoglobulin like receptor, two Ig d; KIR2DL1; NKAT; KIR2DS1; KIR2DS3; NKAT7; KIR2DS5; NKAT9; CD158g; CD158h; CD158a; anti-CD158agh antibody
Reactivity
Human
Isotype
Mouse IgG2b kappa
Clone Number
HP-MA4
Specificity
The mouse monoclonal antibody HP-MA4 recognizes an extracellular epitope of CD158 isoforms KIR2DL1 (CD158a), KIR2DS5 (CD158g), KIR2DS1 (CD158h), and KIR2DS3. It does not recognize the isoforms CD158b1,d,f,i,j.
Form/Format
PE-Cy7
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffe
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffe
Applicable Applications for anti-CD158agh antibody
FCM/FACS (Flow Cytometry)
Immunogen
Human NK cell line LB2
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human CD158agh positive CD3 negative lymphocytes (red-filled) from CD158agh negative CD3 positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD158agh (HP-MA4) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human CD158agh positive CD3 negative lymphocytes (red-filled) from CD158agh negative CD3 positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD158agh (HP-MA4) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD3 (UCHT1) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood) and anti-human CD158agh (HP-MA4) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD3 (UCHT1) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood) and anti-human CD158agh (HP-MA4) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD158agh (HP-MA4) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD158agh (HP-MA4) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
Related Product Information for anti-CD158agh antibody
Killer cell immunoglobulin-like receptors (KIRs) are polymorphic transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. They are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain (such as CD158a/KIR2DL1) transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain (such as CD158g/KIR2DS5, CD158h/KIR2DS1, or KIR2DS3) lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for CD158 isoforms are subsets of MHC class I molecules.
References
*Wiernik A, Foley B, Zhang B, Verneris MR, Warlick E, Gleason MK, Ross JA, Luo X, Weisdorf DJ, Walcheck B, Vallera DA, Miller JS: Targeting natural killer cells to acute myeloid leukemia in vitro with a CD16 x 33 bispecific killer cell engager and ADAM17 inhibition. Clin Cancer Res. 2013 Jul 15;19(14):3844-55, URL: https://pubmed.ncbi.nlm.nih.gov/23690482/,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
24,917 Da
NCBI Official Full Name
Killer cell immunoglobulin-like receptor 2DL4
NCBI Official Synonym Full Names
killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4
NCBI Official Symbol
KIR2DL4
NCBI Official Synonym Symbols
G9P; CD158D; KIR103; KIR-2DL4; KIR103AS; KIR-103AS
NCBI Protein Information
killer cell immunoglobulin-like receptor 2DL4
UniProt Protein Name
Killer cell immunoglobulin-like receptor 2DL4
UniProt Gene Name
KIR2DL4
UniProt Synonym Gene Names
CD158D; KIR103AS; KIR-103AS
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Product Notes
The CD158agh kir2dl4 (Catalog #AAA129298) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD158agh PE-Cy7 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD158agh can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD158agh kir2dl4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD158agh, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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