anti-Human CD1b Antibody | anti-CD1b antibody

Anti-Hu CD1b APC

Cat. Num. AAA129287

• Gene Names: CD1B; R1; CD1; CD1A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD1b; N/A; Anti-Hu CD1b APC; CD1b molecule; R1; anti-CD1b antibody

• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Clone Number: SN13
• Specificity: The mouse monoclonal antibody SN13 (also known as K5-1B8) recognizes an extracellular epitope of CD1b, a 44 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells.
• Form/Format: APC; Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m

• Applicable Applications for anti-CD1b antibody: FCM/FACS (Flow Cytometry)
• Immunogen: A cell membrane antigen preparation that was isolated from normal human thymocytes
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD11c positive CD1b positive cells (red-filled) from CD11c negative CD1b negative cells (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1b (SN13) APC antibody (10 ?l reagent per milion cells in 100 ?l of cell suspension).)

FCM/FACS (Flow Cytometry)

(Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells using anti-human CD1b (SN13) APC antibody (10 ?l reagent per milion cells in 100 ?l of cell suspension) and anti-human CD11c (BU15) PE antibody (20 ?l reagent per milion cells in 100 ?l of cell suspension).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1b (SN13) APC antibody (10 ?l reagent per milion cells in 100 ?l of cell suspension).)

Related Product Information for anti-CD1b antibody

CD1b (also known as R1) together with CD1a and c, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. Besides non-peptide glycolipid antigen presentation to CD1-restricted T cells, CD1b has been implicated in thymocyte development.

References

*Hayes SM, Knight KL: Group 1 CD1 genes in rabbit. J Immunol. 2001 Jan 1;166(1):403-10., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=hayes+sm+knight+kl,*Tentori L, Graziani G, Porcelli SA, Sugita M, Brenner MB, Madaio R, Bonmassar E, Giuliani A, Aquino A: Rifampin increases cytokine-induced expression of the CD1b molecule in human peripheral blood monocytes. Antimicrob Agents Chemother. 1998 Mar;42(3):550-4., URL: http://www.ncbi.nlm.nih.gov/pubmed/9517931,*Giuliani A, Prete SP, Graziani G, Aquino A, Balduzzi A, Sugita M, Brenner MB, Iona E, Fattorini L, Orefici G, Porcelli SA, Bonmassar E: Influence of Mycobacterium bovis bacillus Calmette Guérin on in vitro induction of CD1 molecules in human adherent mononuclear cells. Infect Immun. 2001 Dec;69(12):7461-70., URL: http://www.ncbi.nlm.nih.gov/pubmed/11705921,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL:,

NCBI and Uniprot Product Information

• NCBI GI #: 4502645
• NCBI GeneID: 910
• NCBI Accession #: NP_001755.1
• NCBI GenBank Nucleotide #: NM_001764.2
• UniProt Accession #: P29016; Q5TDK9; Q5TDL0; Q9UMM2
• Molecular Weight: 30,740 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD1b
• NCBI Official Synonym Full Names: CD1b molecule
• NCBI Official Symbol: CD1B
• NCBI Official Synonym Symbols: R1; CD1; CD1A
• NCBI Protein Information: T-cell surface glycoprotein CD1b; CD1B antigen, b polypeptide; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3
• UniProt Protein Name: T-cell surface glycoprotein CD1b
• UniProt Gene Name: CD1B
• UniProt Entry Name: CD1B_HUMAN