anti-Human CD28 Antibody | anti-CD28 antibody

Anti-CD28 [9.3]

Cat. Num. AAA72407

• Gene Names: CD28; Tp44
• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Immunofluorescence
• Purity: Purified. Protein A affinity purified
• Synonyms: CD28; N/A; Anti-CD28 [9.3]; T-cell-specific surface glycoprotein CD28; TP44; anti-CD28 antibody

• Reactivity: Human
• Isotype: Rabbit IgG kappa
• Clone Number: 9.3
• Specificity: The antibody is specific for human CD28. CD28 is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins.
• Purity/Purification: Purified. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD28 antibody: IP (Immunoprecipitation), ELISA, Blocking, IF (Immunofluorescence)
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

IF (Immunofluorescence)

(Immunofluorescence staining of Jurkat cells with anti-CD28 antibody 9.3 . Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 9.3 (AAA72407) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72407, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)

FCM/FACS (Flow Cytometry)

(Flow cytometry using anti-CD28 antibody 9.3 . Jurkat cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 9.3 (AAA72407, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.)

Related Product Information for anti-CD28 antibody

The original antibody was generated by immunizing mice with human T cells.
The original paper describes the generation and characterization of the antibody.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Product Categories/Family for anti-CD28 antibody

GVHD; autoimmune disease

References

Hansen et al.
Immunogenetics. 1980; 10:247.

NCBI and Uniprot Product Information

• NCBI GI #: 340545508
• NCBI GeneID: 940
• NCBI Accession #: NP_001230006.1
• NCBI GenBank Nucleotide #: NM_001243077.1
• UniProt Accession #: P10747; Q13964; Q52M23; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
• Molecular Weight: 25,066 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 2
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28; CD28 antigen
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_HUMAN