FCM/FACS (Flow Cytometry)
(Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD300e (UP-H2) FITC antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
anti-Human CD300e Antibody | anti-CD300e antibody
Anti-Hu CD300e FITC
Gene Names
CD300E; CLM2; CLM-2; IREM2; PIgR2; IREM-2; PIgR-2; CD300LE; CMRF35-A5
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD300e; N/A; Anti-Hu CD300e FITC; CD300e molecule; CLM2; CMRF35-A5; LMIR6; IREM-2; PlgR2; anti-CD300e antibody
Reactivity
Human
Isotype
Mouse IgG1 kappa
Clone Number
UP-H2
Specificity
The mouse monoclonal antibody UP-H2 recognizes an extracellular epitope on CD300e/IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.
Form/Format
FITC
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 1
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 1
Applicable Applications for anti-CD300e antibody
FCM/FACS (Flow Cytometry)
Immunogen
CD300e-HA-transfected cells
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD300e (UP-H2) FITC antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD300e (UP-H2) FITC antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD300e (UP-H2) FITC antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD300e (UP-H2) FITC antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
Related Product Information for anti-CD300e antibody
CD300e/IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.
References
*Aguilar H, Alvarez-Errico D, García-Montero AC, Orfao A, Sayós J, López-Botet M: Molecular characterization of a novel immune receptor restricted to the monocytic lineage. J Immunol. 2004 Dec 1;173(11):6703-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=aguilar+h+orfao+a,*Brckalo T, Calzetti F, Pérez-Cabezas B, Borràs FE, Cassatella MA, López-Botet M: Functional analysis of the CD300e receptor in human monocytes and myeloid dendritic cells. Eur J Immunol. 2010 Mar;40(3):722-32., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=brckalo+t+calzetti,
*Clark GJ, Ju X, Tate C, Hart DN: The CD300 family of molecules are evolutionarily significant regulators of leukocyte functions. Trends Immunol. 2009 May;30(5):209-17., URL: http://www.ncbi.nlm.nih.gov/pubmed/19359216,
*Clark GJ, Ju X, Tate C, Hart DN: The CD300 family of molecules are evolutionarily significant regulators of leukocyte functions. Trends Immunol. 2009 May;30(5):209-17., URL: http://www.ncbi.nlm.nih.gov/pubmed/19359216,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,918 Da
NCBI Official Full Name
CMRF35-like molecule 2
NCBI Official Synonym Full Names
CD300e molecule
NCBI Official Symbol
CD300E
NCBI Official Synonym Symbols
CLM2; CLM-2; IREM2; PIgR2; IREM-2; PIgR-2; CD300LE; CMRF35-A5
NCBI Protein Information
CMRF35-like molecule 2
UniProt Protein Name
CMRF35-like molecule 2
UniProt Gene Name
CD300E
UniProt Synonym Gene Names
CD300LE; CLM2; CMRF35A5; IREM2; CLM-2; IREM-2; PIgR-2; PIgR2; Poly-Ig receptor 2
UniProt Entry Name
CLM2_HUMAN
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Product Notes
The CD300e cd300e (Catalog #AAA129270) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD300e FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD300e can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD300e cd300e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD300e, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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