IF (Immunofluorescence)
(Immunofluorescence staining of Kelly cells with anti-Disialoganglioside 2 antibody 14G2a . Immunofluorescence analysis of paraformaldehyde fixed Kelly cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 14G2a (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Human Disialoganglioside 2 Antibody
Anti-Disialoganglioside 2 [14G2a]
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Purified. Protein A affinity purified
Synonyms
Disialoganglioside 2; N/A; Anti-Disialoganglioside 2 [14G2a]; GD2; anti-Disialoganglioside 2 antibody
Reactivity
Human
Isotype
Mouse IgG2a kappa
Clone Number
14G2a
Specificity
This antibody binds the GD2 antigen and GD2 expressing neuroblastoma cell lines with an affinity similar to the parent antibody.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Disialoganglioside 2 antibody
FCM/FACS (Flow Cytometry), ELISA
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of Kelly cells with anti-Disialoganglioside 2 antibody 14G2a . Immunofluorescence analysis of paraformaldehyde fixed Kelly cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 14G2a (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of Kelly cells with anti-Disialoganglioside 2 antibody 14G2a . Immunofluorescence analysis of paraformaldehyde fixed Kelly cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 14G2a (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-Disialoganglioside 2 antibody 14G2a . Kelly cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 14G2a at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-Disialoganglioside 2 antibody 14G2a . Kelly cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 14G2a at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-Disialoganglioside 2 antibody
The original murine parent antibody was generated against human neuroblastoma cell line LAN-1. This IgG2a isotype version was then generated by class switching of the murine parent antibody 14.18 (IgG3).
Describes the generation of isotype variants of murine parent antibody 14.18.
Describes the generation of isotype variants of murine parent antibody 14.18.
Product Categories/Family for anti-Disialoganglioside 2 antibody
References
Mujoo et al.
Cancer Res. (1989); 49(11):2857-61.
Cancer Res. (1989); 49(11):2857-61.
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Product Notes
The Disialoganglioside 2 (Catalog #AAA72310) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Disialoganglioside 2 [14G2a] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Disialoganglioside 2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the Disialoganglioside 2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Disialoganglioside 2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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