WB (Western Blot)
(Western blot using anti-EGFRvIII antibody 806 . A431 (A) (0.003ug/ml), HeLa (B) (0.3ug/ml), and MDA-MB-231 (C) (0.1ug/ml) cells lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 806 (AAA72335) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
anti-Human EGFRvIII Antibody | anti-EGFRvIII antibody
Anti-EGFRvIII [806]
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Purified. Protein A affinity purified
Synonyms
EGFRvIII; N/A; Anti-EGFRvIII [806]; de2-7 EGFR; EGF receptor; delta2-7 epidermal growth factor receptor; Epidermal Growth Factor Receptor variant III; anti-EGFRvIII antibody
Reactivity
Human
Isotype
Rabbit IgG kappa
Clone Number
806
Specificity
This antibody is specific for the human delta2-7 epidermal growth factor receptor (de2-7 EGFR or EGFRvIII) which is a truncated version of the EGFR commonly expressed in glioma. This antibody does not bind wild type EGF receptor. Studies have shown that it binds a cryptic epitope on the EGFR in the region between amino acids 287-302, and it is suggested that breaking the disulfide bond preceding the epitope might allow the CR1 domain to open up sufficiently for antibody binding.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-EGFRvIII antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western blot using anti-EGFRvIII antibody 806 . A431 (A) (0.003ug/ml), HeLa (B) (0.3ug/ml), and MDA-MB-231 (C) (0.1ug/ml) cells lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 806 (AAA72335) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
WB (Western Blot)
(Western blot using anti-EGFRvIII antibody 806 . A431 (A) (0.003ug/ml), HeLa (B) (0.3ug/ml), and MDA-MB-231 (C) (0.1ug/ml) cells lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 806 (AAA72335) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
IF (Immunofluorescence)
(Immunofluorescence staining of A431 cells with anti-EGFRvIII antibody 806 . Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 806 (AAA72335) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cell junction staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72335, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of A431 cells with anti-EGFRvIII antibody 806 . Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 806 (AAA72335) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cell junction staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72335, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-EGFRvIII antibody
The original antibody was generated by immunization of mice with NR6 mouse fibroblasts expressing the truncated de2-7 EGFR.
Describes the generation of this antibody and examines the antitumor activity of this antibody against human tumor xenografts grown in nude mice.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Describes the generation of this antibody and examines the antitumor activity of this antibody against human tumor xenografts grown in nude mice.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Product Categories/Family for anti-EGFRvIII antibody
References
Luwor et al.
Cancer Res. 2001 Jul 15;61(14):5355-61.
Cancer Res. 2001 Jul 15;61(14):5355-61.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
134,277 Da
NCBI Official Full Name
epidermal growth factor receptor isoform a
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61
NCBI Protein Information
epidermal growth factor receptor; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
UniProt Entry Name
EGFR_HUMAN
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Product Notes
The EGFRvIII egfr (Catalog #AAA72335) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-EGFRvIII [806] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EGFRvIII can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the EGFRvIII egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFRvIII, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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