IF (Immunofluorescence)
(Immunofluorescence staining of Daudi cells with anti-HLA-A2 antibody BB7.2 . Immunofluorescence analysis of paraformaldehyde-fixed Daudi cells on Shi-fix™ coverslips stained with the mouse IgG2b version of BB7.2 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Human HLA-A2 Antibody | anti-HLA-A2 antibody
Anti-HLA-A2 [BB7.2]
IF (Immunofluorescence)
(Immunofluorescence staining of Daudi cells with anti-HLA-A2 antibody BB7.2 . Immunofluorescence analysis of paraformaldehyde-fixed Daudi cells on Shi-fix™ coverslips stained with the mouse IgG2b version of BB7.2 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of Daudi cells with anti-HLA-A2 antibody BB7.2 . Immunofluorescence analysis of paraformaldehyde-fixed Daudi cells on Shi-fix™ coverslips stained with the mouse IgG2b version of BB7.2 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western blot using anti-HLA-A2 antibody BB7.2 . Daudi cells lysate (35ug protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of BB7.2 (AAA72323) at 0.3ug/ml before detection using an appropriate secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
WB (Western Blot)
(Western blot using anti-HLA-A2 antibody BB7.2 . Daudi cells lysate (35ug protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of BB7.2 (AAA72323) at 0.3ug/ml before detection using an appropriate secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
IF (Immunofluorescence)
(Immunofluorescence staining of Daudi cells with anti-HLA-A2 antibody BB7.2 . Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of BB7.2 (AAA72323) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72323, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of Daudi cells with anti-HLA-A2 antibody BB7.2 . Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of BB7.2 (AAA72323) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72323, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western blot using anti-HLA-A2 antibody BB7.2 . Daudi cells lysate (35ug protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of BB7.2 (AAA72323) at 0.3ug/ml before detection using an appropriate secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
WB (Western Blot)
(Western blot using anti-HLA-A2 antibody BB7.2 . Daudi cells lysate (35ug protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of BB7.2 (AAA72323) at 0.3ug/ml before detection using an appropriate secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
Describes the generation and purification of this antibody.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Hum Immunol. 1981 Dec;3(4):277-99.
NCBI and Uniprot Product Information
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Product Notes
The HLA-A2 hla-a (Catalog #AAA72323) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-HLA-A2 [BB7.2] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HLA-A2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the HLA-A2 hla-a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA-A2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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