FCM/FACS (Flow Cytometry)
(Separation of human CD17A positive CD3 positive lymphocytes (red-filled) from CD17A negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-17A (9F9) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
anti-Human IL-17A Antibody | anti-IL-17A antibody
Anti-Hu IL-17A APC
Gene Names
IL17A; IL17; CTLA8; IL-17; IL-17A
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
IL-17A; N/A; Anti-Hu IL-17A APC; interleukin 17A; Interleukin 17; anti-IL-17A antibody
Reactivity
Human
Isotype
Mouse IgG1 kappa
Clone Number
9F9
Specificity
The mouse monoclonal antibody 9F9 recognizes human interleukin 17A (IL-17A; secreted or intracellular).
Form/Format
APC
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m
Applicable Applications for anti-IL-17A antibody
FCM/FACS (Flow Cytometry)
Immunogen
mammalian-derived human IL-17-IgG fusion protein, boost with recombinant human IL-17A
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human CD17A positive CD3 positive lymphocytes (red-filled) from CD17A negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-17A (9F9) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human CD17A positive CD3 positive lymphocytes (red-filled) from CD17A negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-17A (9F9) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining pattern of PHA stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) PE antibody (20 ?l reagent / 100 ?l of peripheral whole blood) and intracellular staining using anti-human IL-17A (9F9) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining pattern of PHA stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) PE antibody (20 ?l reagent / 100 ?l of peripheral whole blood) and intracellular staining using anti-human IL-17A (9F9) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry intracellular staining pattern of PHA stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-17A (9F9) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry intracellular staining pattern of PHA stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-17A (9F9) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
Related Product Information for anti-IL-17A antibody
Interleukin 17A (IL-17A) is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. It plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. It is the signature effector cytokine of Th17 cells, and in this role it primarily induces neutrophil activation and recruitment at infection and inflammatory sites. High levels of IL-17A are associated with rheumatoid arthritis, psoriasis, multiple sclerosis, and another inflammatory diseases, including lung injugy during severe COVID 19. This cytokine also contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. It is an effector cytokine for invariant NKT cells (iNKT), and it is involved in epithelial barrier formation upon injury.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,504 Da
NCBI Official Full Name
interleukin-17A
NCBI Official Synonym Full Names
interleukin 17A
NCBI Official Symbol
IL17A
NCBI Official Synonym Symbols
IL17; CTLA8; IL-17; IL-17A
NCBI Protein Information
interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
UniProt Protein Name
Interleukin-17A
UniProt Gene Name
IL17A
UniProt Synonym Gene Names
CTLA8; IL17; IL-17; IL-17A; CTLA-8
UniProt Entry Name
IL17_HUMAN
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Product Notes
The IL-17A il17a (Catalog #AAA129212) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu IL-17A APC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-17A can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the IL-17A il17a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-17A, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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