IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Modified cytokeratin antibody COU-1 . Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of COU-1 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Human Modified cytokeratin Antibody | anti-KRT8 antibody
Anti-Modified cytokeratin [COU-1]
Gene Names
KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Purified using a recombinant lectin column
Synonyms
Modified cytokeratin; N/A; Anti-Modified cytokeratin [COU-1]; KRT8; CYK8; Keratin type II cytoskeletal 8; Cytokeratin-8; Type-II keratin Kb8; KRT18; CYK18; Keratin type I cytoskeletal 18; Cytokeratin-18; Cell proliferation-inducing gene 46 protein; COU1; B9165; ECACC 87040201; anti-KRT8 antibody
Reactivity
Human
Isotype
Human IgM kappa
Clone Number
COU-1
Specificity
This antibody binds modified forms of human cytokeratin 8 and cytokeratin 18. It is reported that this antibody recognizes an intracellularly located cytokeratin protein that is strongly expressed in adenocarcinomas.
Purity/Purification
Affinity Purified using a recombinant lectin column
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-KRT8 antibody
ELISA, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Modified cytokeratin antibody COU-1 . Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of COU-1 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Modified cytokeratin antibody COU-1 . Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of COU-1 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-Modified cytokeratin antibody COU-1 . Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of COU-1 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-Modified cytokeratin antibody COU-1 . Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of COU-1 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-KRT8 antibody
The original antibody was generated by fusing the human lymphoblastoid cell line WI-L2-729-HF2 with lymphocytes obtained from mesenteric lymph nodes from a colon cancer patient.
Describes the generation of a human-human hybridoma with lymphocytes from patients with colorectal cancer.
Describes the generation of a human-human hybridoma with lymphocytes from patients with colorectal cancer.
Product Categories/Family for anti-KRT8 antibody
References
Christensen et al.
Int J Cancer. 1986 May 15;37(5):683-8.
Int J Cancer. 1986 May 15;37(5):683-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,704 Da
NCBI Official Full Name
keratin, type II cytoskeletal 8 isoform 2
NCBI Official Synonym Full Names
keratin 8
NCBI Official Symbol
KRT8
NCBI Official Synonym Symbols
K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
NCBI Protein Information
keratin, type II cytoskeletal 8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8
UniProt Protein Name
Keratin, type II cytoskeletal 8
UniProt Gene Name
KRT8
UniProt Synonym Gene Names
CYK8; CK-8; K8
UniProt Entry Name
K2C8_HUMAN
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Product Notes
The KRT8 krt8 (Catalog #AAA72380) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Modified cytokeratin [COU-1] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Modified cytokeratin can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the KRT8 krt8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Modified cytokeratin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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