anti-Human SCIMP Antibody | anti-SCIMP antibody

Anti-SCIMP PE

Cat. Num. AAA129201

• Gene Names: SCIMP; UNQ5783; C17orf87
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: SCIMP; N/A; Anti-SCIMP PE; SLP adaptor and CSK interacting membrane protein; NVL; anti-SCIMP antibody

• Reactivity: Human
• Isotype: Mouse IgG2a
• Clone Number: NVL-07
• Specificity: The mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.
• Form/Format: PE; Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Applicable Applications for anti-SCIMP antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant intracellular part of human SCIMP
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human HLA-DR/DP positive SCIMP positive lymphocytes (red-filled) from HLA-DR/DP negative SCIMP negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-SCIMP (NVL-07) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry multicolor staining pattern of human lymphocytes using anti-human HLA-DR/DP (MEM-136) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood, surface staining) and anti-SCIMP (NVL-07) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood, intracellular staining).)

FCM/FACS (Flow Cytometry)

(Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-SCIMP (NVL-07) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

Related Product Information for anti-SCIMP antibody

SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.

References

*Draber P, Vonkova I, Stepanek O, Hrdinka M, Kucova M, Skopcova T, Otahal P, Angelisova P, Horejsi V, Yeung M, Weiss A, Brdicka T: SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling. Mol Cell Biol. 2011 Nov;31(22):4550-62., URL: https://pubmed.ncbi.nlm.nih.gov/21930792/,

NCBI and Uniprot Product Information

• NCBI GI #: 427197601
• NCBI GeneID: 388325
• NCBI Accession #: NP_001258771.1
• NCBI GenBank Nucleotide #: NM_001271842.1
• UniProt Accession #: Q6UWF3; Q96MD0; A6XGL4; B4DLK1
• Molecular Weight: 15,693 Da
• NCBI Official Full Name: SLP adapter and CSK-interacting membrane protein isoform 2
• NCBI Official Synonym Full Names: SLP adaptor and CSK interacting membrane protein
• NCBI Official Symbol: SCIMP
• NCBI Official Synonym Symbols: UNQ5783; C17orf87
• NCBI Protein Information: SLP adapter and CSK-interacting membrane protein
• UniProt Protein Name: SLP adapter and CSK-interacting membrane protein
• UniProt Gene Name: SCIMP
• UniProt Synonym Gene Names: C17orf87
• UniProt Entry Name: SCIMP_HUMAN