FCM/FACS (Flow Cytometry)
(Expression profiling on peripheral blood subsets using Anti-SIGLEC10 PE antibody (clone 5G6).HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of anti-human SIGLEC10 PE antibody (clone 5G6, 10 ?l reagent / 100 ?l of stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) were added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 ul of PBS for acquisition.)
anti-Human SIGLEC10 Antibody | anti-SIGLEC10 antibody
Anti-Hu SIGLEC10 PE
Gene Names
SIGLEC10; SLG2; PRO940; SIGLEC-10
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
SIGLEC10; N/A; Anti-Hu SIGLEC10 PE; sialic acid binding Ig like lectin 10; PRO940; SGL2; anti-SIGLEC10 antibody
Reactivity
Human
Isotype
Mouse IgG1
Clone Number
5G6
Specificity
The mouse monoclonal antibody 5G6 recognizes an extracellular epitope of human SIGLEC10, a sialic acid-binding lectin expressed on subsets of human leucocytes.
Form/Format
PE
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Applicable Applications for anti-SIGLEC10 antibody
FCM/FACS (Flow Cytometry)
Immunogen
SIGLEC10 extracellular domain fused with human IgG1 Fc fragment
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Expression profiling on peripheral blood subsets using Anti-SIGLEC10 PE antibody (clone 5G6).HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of anti-human SIGLEC10 PE antibody (clone 5G6, 10 ?l reagent / 100 ?l of stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) were added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 ul of PBS for acquisition.)
FCM/FACS (Flow Cytometry)
(Expression profiling on peripheral blood subsets using Anti-SIGLEC10 PE antibody (clone 5G6).HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of anti-human SIGLEC10 PE antibody (clone 5G6, 10 ?l reagent / 100 ?l of stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) were added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 ul of PBS for acquisition.)
FCM/FACS (Flow Cytometry)
(Anti-SIGLEC10 PE antibody (clone 5G6) works in flow cytometry application.Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Mouse monoclonal anti-human SIGLEC10 PE antibody (clone 5G6) was used in amount of 10 ul in 100 ul of blood sample (2 x 106 cells).)
FCM/FACS (Flow Cytometry)
(Anti-SIGLEC10 PE antibody (clone 5G6) works in flow cytometry application.Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Mouse monoclonal anti-human SIGLEC10 PE antibody (clone 5G6) was used in amount of 10 ul in 100 ul of blood sample (2 x 106 cells).)
Related Product Information for anti-SIGLEC10 antibody
SIGLEC10 is a CD33-related receptor of sialoglycans, expressed on eosinophils, monocytes, a subpopulation of NK cells, and at lower level on B cells. Its murine ortholog is Siglec G. SIGLEC10 seems to act as an immunomodulatory receptor, which binds to VAP-1, a glycoprotein expressed on endothelium under inflammatory conditions. Another ligand of SIGLEC10 is CD24, a marker of poorer prognosis in carcinomas.
References
*Stephenson HN, Mills DC, Jones H, Milioris E, Copland A, Dorrell N, Wren BW, Crocker PR, Escors D, Bajaj-Elliott M: Pseudaminic acid on Campylobacter jejuni flagella modulates dendritic cell IL-10 expression via Siglec-10 receptor: A novel flagellin-host interaction. J Infect Dis. 2014 Nov 1;210(9):1487-98., URL: https://pubmed.ncbi.nlm.nih.gov/24823621/,*Nguyen DH, Ball ED, Varki A: Myeloid precursors and acute myeloid leukemia cells express multiple CD33-related Siglecs. Exp Hematol. 2006 Jun;34(6):728-35., URL: https://pubmed.ncbi.nlm.nih.gov/16728277/,*Läubli H, Kawanishi K, Vazhappilly CG, Matar R, Merheb M, Siddiqui SS: Tools to study and target the Siglec-sialic acid axis in cancer. FEBS J. 2021 Nov;288(21):6206-6225., URL: https://pubmed.ncbi.nlm.nih.gov/33251699/,*Bhamidipati K, Silberstein JL, Chaichian Y, Baker MC, Lanz TV, Zia A, Rasheed YS, Cochran JR, Robinson WH: CD52 is elevated on B cells of SLE patients and regulates B cell function. Front Immunol. 2021 Feb 4;11:626820., URL: https://pubmed.ncbi.nlm.nih.gov/33658999/,*Munday J, Kerr S, Ni J, Cornish AL, Zhang JQ, Nicoll G, Floyd H, Mattei MG, Moore P, Liu D, Crocker PR: Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor. Biochem J. 2001 Apr 15;355(Pt 2):489-97., URL: https://pubmed.ncbi.nlm.nih.gov/11284738/,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sialic acid-binding Ig-like lectin 10 isoform 2
NCBI Official Synonym Full Names
sialic acid binding Ig like lectin 10
NCBI Official Symbol
SIGLEC10
NCBI Official Synonym Symbols
SLG2; PRO940; SIGLEC-10
NCBI Protein Information
sialic acid-binding Ig-like lectin 10
UniProt Protein Name
Sialic acid-binding Ig-like lectin 10
UniProt Gene Name
SIGLEC10
UniProt Synonym Gene Names
SLG2; Siglec-10
UniProt Entry Name
SIG10_HUMAN
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Product Notes
The SIGLEC10 siglec10 (Catalog #AAA129200) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu SIGLEC10 PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIGLEC10 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the SIGLEC10 siglec10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIGLEC10, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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