anti-Human TLR6 Antibody | anti-TLR6 antibody

Goat anti-TLR6 Antibody

Cat. Num. AAA62020

• Reactivity: Human
• Applications: Immunofluorescence, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TLR6; N/A; Goat anti-TLR6 Antibody; CD286; OTTHUMP00000158730; toll-like receptor 6; anti-TLR6 antibody

• Reactivity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: C-RGSTLLNFTLNH

• Applicable Applications for anti-TLR6 antibody: IF (Immunofluorescence), ELISA (Peptide ELISA)
• Epitope: internal region
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of paraformaldehyde fixed THP-1 cells (blue line) permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488® conjugated secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic (endoplasmic reticulum) staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

References

Davila S, Froeling FE, Tan A, Bonnard C, Boland GJ, Snippe H, Hibberd ML, Seielstad M, New genetic associations detected in a host response study to hepatitis B vaccine. Genes and immunity 2010 Mar : . PMID: 20237496

NCBI and Uniprot Product Information

• NCBI GeneID: 10333