WB (Western Blot)
(All lanes: MCF-7 Whole Cell Lysate (40ug)Lane 1: Anti-GAPDH antibodyLane 2: Anti-Beta tubulin antibody)
MCF-7 cell lysate
MCF-7
Applications
Western Blot
Synonyms
MCF-7; N/A; MCF-7 cell lysate
Host
Human
Form/Format
Liquid; In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue).
Concentration
2mg/ml (determined by BCA assay) (varies by lot)
Applicable Applications for MCF-7 cell lysate
WB (Western Blot)
Quality Control
Western Blot analyzed by both GAPDH and Beta tubulin antibody .
Preparation Before Usage
Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Preparation and Storage
Aliquot the lysate into single tube and then storing them -80 degree C or below. Avoid repeated freezing and thawing cycles.
WB (Western Blot)
(All lanes: MCF-7 Whole Cell Lysate (40ug)Lane 1: Anti-GAPDH antibodyLane 2: Anti-Beta tubulin antibody)
WB (Western Blot)
(All lanes: MCF-7 Whole Cell Lysate (40ug)Lane 1: Anti-GAPDH antibodyLane 2: Anti-Beta tubulin antibody)
Related Product Information for MCF-7 cell lysate
MCF-7 cells are breast cancer cell line isolated in 1970 from a 69 year old woman, in 1970s. MCF-7 cells are a well characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin, endothelin, GFAP, neurofilament, vimentin.
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Product Notes
The MCF-7 (Catalog #AAA48307) is a Cell Lysate produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MCF-7 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the MCF-7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCF-7, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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