Carbonic Anhydrase IX / CA9 Active Protein | CA9 active protein

Recombinant Human Carbonic Anhydrase IX / CA9 Protein (His tag)

Cat. Num. AAA173576

• Gene Names: CA9; MN; CAIX
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Carbonic Anhydrase IX / CA9; N/A; Recombinant Human Carbonic Anhydrase IX / CA9 Protein (His tag); CA9, CAIX, MN; CA9 active protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 459

• Species: Human
• Expression host: HEK293 Cells
• Sequence: Met 1-Asp 414
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Tag: C-His
• Bio Activity: Measured by its esterase activity. The specific activity is >30 pmoles/min/ug, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 ug enzyme at 400 nm in 100 uL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70°C.Store itunder sterile conditions at -20°C to -80°C. It is recommended that the protein bealiquoted for optimal storage. Avoid repeated freeze-thaw cycles

SDS-PAGE

Related Product Information for CA9 active protein

Background: Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3-) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.

Description: A DNA sequence encoding the human carbonic anhydrase IX (CA9) precursor (NP_001207.2) (Met 1-Asp 414) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 169636420
• NCBI GeneID: 768
• NCBI Accession #: NP_001207.2
• NCBI GenBank Nucleotide #: NM_001216.2
• UniProt Accession #: Q16790; Q5T4R1
• Molecular Weight: Mol Mass: 42.5 kDa; AP Mol Mass: 48 kDa
• NCBI Official Full Name: carbonic anhydrase 9
• NCBI Official Synonym Full Names: carbonic anhydrase 9
• NCBI Official Symbol: CA9
• NCBI Official Synonym Symbols: MN; CAIX
• NCBI Protein Information: carbonic anhydrase 9
• UniProt Protein Name: Carbonic anhydrase 9
• UniProt Gene Name: CA9
• UniProt Synonym Gene Names: G250; MN; CA-IX; CAIX; RCC-associated antigen G250