SDS-PAGE
HER3 / ErbB3 active protein
Recombinant Human HER3 / ErbB3 Protein (His tag)
Gene Names
ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
Purity
> 95 % as determined by SDS-PAGE
Synonyms
HER3 / ErbB3; N/A; Recombinant Human HER3 / ErbB3 Protein (His tag); HER3, LCCS2, ErbB-3, c-erbB3, erbB3-S, MDA-BF-1, MGC88033, c-erbB-3, p180-ErbB3, p45-sErbB3, p85-sErbB3; HER3 / ErbB3 active protein
Host
Human Cells
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween80.
Sequence Length
1342
Source
Human
Predicted N Terminal
Ser 20
Bio-Activity
1. Measured by its binding ability in a functional ELISA.
2. Immobilized human ErbB3 at 2 ug/mL (100 ul/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.43 ug/mL.
2. Immobilized human ErbB3 at 2 ug/mL (100 ul/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.43 ug/mL.
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Reconstitution
It is recommended that sterile water (70 ul) be added to the vial to prepare a stock solution of 0.15 mg/mL.
Concentration is measured by UV-Vis.
Concentration is measured by UV-Vis.
Preparation and Storage
Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for HER3 / ErbB3 active protein
Background: ErbB3, also known as Her3 (human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
Description: A DNA sequence encoding the extracellular domain (Met 1-Thr 643) of human ErbB3 (NP_001973.2) precursor was expressed, with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the extracellular domain (Met 1-Thr 643) of human ErbB3 (NP_001973.2) precursor was expressed, with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
77,426 Da
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-3 isoform 1
NCBI Official Synonym Full Names
erb-b2 receptor tyrosine kinase 3
NCBI Official Symbol
ERBB3
NCBI Official Synonym Symbols
HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
NCBI Protein Information
receptor tyrosine-protein kinase erbB-3
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-3
UniProt Gene Name
ERBB3
UniProt Synonym Gene Names
HER3
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Product Notes
The HER3 / ErbB3 erbb3 (Catalog #AAA173572) is an Active Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HER3 / ErbB3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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