HLA Class II Histocompatibility Antigen gamma Chain Active Protein | CD74 active protein

Recombinant Mouse HLA Class II Histocompatibility Antigen gamma Chain Protein

Cat. Num. AAA174708

• Gene Names: CD74; II; p33; DHLAG; HLADG; Ia-GAMMA
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: HLA Class II Histocompatibility Antigen gamma Chain; N/A; Recombinant Mouse HLA Class II Histocompatibility Antigen gamma Chain Protein; Cluster of Differentiation 74; CD74 antigen; CD74 molecule, major histocompatibility complex, class II invariant chain; DHLAG gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ia-GAMMA; MHC HLA-DR gamma chain; CD74; DHLAG; Ia-gamma; INVG34;; CD74 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
• Sequence: Gln56-Leu215
• Sequence Length: 232

• Species: Mouse
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Fusion Tag: C-6His
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 mug/ml (100 mul/well) can bind biotinylated human CTSL1 with a linear range of 3.2-400 ng/ml.
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents tothe bottom. Do not mix by vortex.It is recommended that sterile water(200ul) be added to the vial to prepare astock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Ships on blue ice. Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for CD74 active protein

Protein Construction: Recombinant Mouse HLA class II histocompatibility antigen gamma chain is produced by our mammalian expression system and the target gene encoding Gln56-Leu215 is expressed with a 6His tag at the C-terminus.

Background: Mouse HLA class II histocompatibility antigen gamma chain (CD74), is a single-pass type II membrane glycoprotein which contains 1 thyroglobulin type-1 domain. Mouse CD74 shares 75% and 88% aa sequence identity with human and rat CD74, respectively. CD74 plays an important role in adaptive immunity, inflammation, and cancer. It plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place.CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up-regulation of angiogenic factors in endometrial stromal cells.

NCBI and Uniprot Product Information

• NCBI GI #: 10835071
• NCBI GeneID: 972
• NCBI Accession #: NP_004346.1
• NCBI GenBank Nucleotide #: NP_004346.1
• UniProt Accession #: P04441-2; P04233
• Molecular Weight: Molecular Mass: 19.4kDa; Actual Protein Molecular Mass: 25-30kDa
• NCBI Official Full Name: HLA class II histocompatibility antigen gamma chain isoform b
• NCBI Official Synonym Full Names: CD74 molecule
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; p33; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain
• UniProt Protein Name: HLA class II histocompatibility antigen gamma chain
• UniProt Gene Name: CD74
• UniProt Synonym Gene Names: DHLAG; Ii
• UniProt Entry Name: HG2A_HUMAN