Standard Curve (Sample)
Human HLA class II histocompatibility antigen gamma chain ELISA Kit | CD74 elisa kit
Human CD74 (HLA class II histocompatibility antigen gamma chain) ELISA kit
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Synonyms
HLA class II histocompatibility antigen gamma chain; N/A; Human CD74 (HLA class II histocompatibility antigen gamma chain) ELISA kit; CD74 elisa kit
Reactivity
Human
Specificity
This kit recognizes natural and some recombinant Human CD74. No significant crossreactivity or interference between Human CD74 and analogues was observed.
Sequence Length
296
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.625-40ng/mL
Sensitivity
Min: 0.375ng/mL; Max: 40ng/mL
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CD74 elisa kit
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human CD74 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human CD74. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human CD74 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human CD74, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD74. The concentration of Human CD74 in samples can be calculated by comparing the OD of the samples with the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human CD74. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human CD74 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human CD74, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD74. The concentration of Human CD74 in samples can be calculated by comparing the OD of the samples with the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,328 Da
NCBI Official Full Name
HLA class II histocompatibility antigen gamma chain isoform a
NCBI Official Synonym Full Names
CD74 molecule
NCBI Official Symbol
CD74
NCBI Official Synonym Symbols
II; DHLAG; HLADG; Ia-GAMMA
NCBI Protein Information
HLA class II histocompatibility antigen gamma chain
UniProt Protein Name
HLA class II histocompatibility antigen gamma chain
UniProt Gene Name
CD74
UniProt Synonym Gene Names
DHLAG; Ii
UniProt Entry Name
HG2A_HUMAN
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Product Notes
The Human CD74 cd74 (Catalog #AAA171415) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA171415 ELISA Kit recognizes Human CD74. It is sometimes possible for the material contained within the vial of "HLA class II histocompatibility antigen gamma chain, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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