Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 Active Protein | CD33L3 active protein

Recombinant Mouse Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-6His)

Cat. Num. AAA177967

• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3; N/A; Recombinant Mouse Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-6His); Angiopoietin-related protein 4; 425O18-1; Angiopoietin-like protein 4; Fasting-induced adipose factor; Hepatic fibrinogen/angiopoietin-related protein; HFARP; Secreted protein Bk89; Angptl4; Farp; Fiaf; Ng27; CD33L3 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, 150mM NaCl, 5% Thehalose, 0.3% Chaps, pH7.4.
• Sequence: Arg24-Thr262

• Species: Mouse
• Tag: C-6His
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Mouse Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Arg24-Thr262 is expressed with a 6His tag at the C-terminus.
• Activity: Immobilized Mouse Siglec-15-His at 2ug/ml (100ul/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 27.7ng/ml.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

Bioactivity

SDS-PAGE

Related Product Information for CD33L3 active protein

Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO--terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts

NCBI and Uniprot Product Information

• UniProt Accession #: A7E1W8
• Molecular Weight: Mol. Mass: 26.5kDa; AP Mol. Mass: 30-40kDa