Standard Curve (Sample)
Human Complement C3 Convertase (C3 Convertase) CLIA Kit | C3 clia kit
High Sensitive Complement C3 Convertase (C3 Convertase) ELISA Kit
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1
Reactivity
Human
Applications
CLIA (Chemiluminescence Immunoassay)
Synonyms
Complement C3 Convertase (C3 Convertase); N/A; High Sensitive Complement C3 Convertase (C3 Convertase) ELISA Kit; C3c; C4b2a; C3bBb; complement C.hivin.4.hivin2; C3 clia kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of C3 Convertase. No significant cross-reactivity or interference between C3 Convertase and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.147ng/mL
Applicable Applications for C3 clia kit
CLIA (Chemiluminescence Immunoassay)
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C3 Convertase were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C3 Convertase were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for C3 clia kit
Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of C3 Convertase in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to C3 Convertase. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to C3 Convertase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the C3 Convertase level in the sample or standard.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to C3 Convertase. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to C3 Convertase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the C3 Convertase level in the sample or standard.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
187,148 Da
NCBI Official Full Name
Complement component 3
NCBI Official Synonym Full Names
complement component 3
NCBI Official Symbol
C3
NCBI Official Synonym Symbols
ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1
NCBI Protein Information
complement C3; prepro-C3; C3a anaphylatoxin; complement component C3; complement component C3a; complement component C3b; acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
UniProt Protein Name
Complement C3
UniProt Gene Name
C3
UniProt Synonym Gene Names
CPAMD1; ASP
UniProt Entry Name
CO3_HUMAN
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Product Notes
The Human C3 c3 (Catalog #AAA189258) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA189258 CLIA Kit recognizes Human C3. AAA Biotech's Complement C3 Convertase (C3 Convertase) can be used in a range of immunoassay formats including, but not limited to, CLIA (Chemiluminescence Immunoassay). Researchers should empirically determine the suitability of the C3 c3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Complement C3 Convertase (C3 Convertase), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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