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product-image-AAA189719_SC15.jpg Standard Curve (Sample)

Human Complement Component 4d (C4d) CLIA Kit | C4d clia kit

Human CLIA Kit for Complement Component 4d (C4d) CLIA Kit

Gene Names
C4B; CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
Reactivity
Human
Synonyms
Complement Component 4d (C4d); N/A; Human CLIA Kit for Complement Component 4d (C4d) CLIA Kit; C4d/Complement Component 4d; C4d clia kit
Ordering
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of C4d. No significant cross-reactivity or interference between C4d and analogues was observed.
Samples
Human serum, plasma and other biological fluids
Detection Range
1.37-1,000pg/mL
Sensitivity
< 0.61pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C4d were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C4d were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 4 degree C for 1 month/-20 degree C for long-term (within shelf life)
Shelf Life: 8 months
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

product-image-AAA189719_SC15.jpg Standard Curve (Sample)
Related Product Information for C4d clia kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to C4d. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to C4d. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the C4d level in the sample or standard.

Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of C4d in human serum, plasma and other biological fluids.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
721
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
192,751 Da
NCBI Official Full Name
complement C4-B preproprotein
NCBI Official Synonym Full Names
complement component 4B (Chido blood group)
NCBI Official Symbol
C4B
NCBI Official Synonym Symbols
CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
NCBI Protein Information
complement C4-B; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; Chido form of C4; basic complement C4; complement C4B1a
UniProt Protein Name
Complement C4-B
UniProt Gene Name
C4B
UniProt Synonym Gene Names
CO4; CPAMD3
UniProt Entry Name
CO4B_HUMAN

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Product Notes

The Human C4d c4b (Catalog #AAA189719) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA189719 CLIA Kit recognizes Human C4d. It is sometimes possible for the material contained within the vial of "Complement Component 4d (C4d), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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