Human EGFR CLIA Kit | EGFR clia kit

EGFR (Human) LumiAb ELISA Kit

Cat. Num. AAA318590

• Gene Names: EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• Reactivity: Human
• Synonyms: EGFR; N/A; EGFR (Human) LumiAb ELISA Kit; Human EGFR; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB1; EGFR clia kit

• Reactivity: Human
• Specificity: The LumiAb Human EGF ELISA is capable of recognizing both recombinant and naturally produced Human EGF proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 1210
• Assay Type: Quantitative Sandwich
• Sensitivity: 4 pg/ml.

• Preparation and Storage: Store at 4 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for EGFR clia kit

Principle of the Assay: The LumiAb Human EGF Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human EGF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human EGF while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human EGF, or Epidermal Growth Factor, is a 1207 amino acid residue protein encoded by the EGF gene located on chromosome 4 at locus 4q25. Typically, defects in this gene are the cause of hypomagnesaemia type 4 and dysregulation of the gene have been associated with the growth and progression of certain cancers. The 1207 amino acid precursor molecule is proteolytically cleaved to generate the 53 residue EGF peptide while removing the 22 residue signal sequence and 1185 residue pro-EGF chain. EGF acts as a potent mitogenic factor via its high affinity binding with the cell surface EGF-Receptor and together, the interaction between the two plays an important role in the growth, proliferation and differentiation of numerous cell types. Further, EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. EGF has magnesiotropic characteristics that stimulate Mg2+ reabsorption in the renal distal convoluted tubule via engagement of EGF-R and activation of the Mg2+ channel, TRPM6.

Product Categories/Family for EGFR clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 29725609
• NCBI GeneID: 1956
• NCBI Accession #: NP_005219.2
• NCBI GenBank Nucleotide #: NM_005228.5
• UniProt Accession #: P00533
• NCBI Official Full Name: epidermal growth factor receptor isoform a
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: EGFR
• NCBI Official Synonym Symbols: ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• NCBI Protein Information: epidermal growth factor receptor
• UniProt Protein Name: Epidermal growth factor receptor
• UniProt Gene Name: EGFR
• UniProt Synonym Gene Names: ERBB; ERBB1; HER1
• UniProt Entry Name: EGFR_HUMAN