Human Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) CLIA Kit | Rac1 clia kit

Human Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)(CLIA) CLIA Kit

Cat. Num. AAA189605

• Gene Names: RAC1; MIG5; Rac-1; TC-25; p21-Rac1
• Reactivity: Human
• Applications: CLIA (Chemiluminescence Immunoassay)
• Synonyms: Ras Related C3 Botulinum Toxin Substrate 1 (Rac1); N/A; Human Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)(CLIA) CLIA Kit; CLIA Kit for Ras Related C3 Botulinum Toxin Substrate 1 (Rac1); Rac1/Ras Related C3 Botulinum Toxin Substrate 1; TC-25; p21-Rac1; MIG5; Cell migration-inducing gene 5 protein; Ras-like protein TC25; Rac1 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rac1. No significant cross-reactivity or interference between Rac1 and analogues was observed.
• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Detection Range: 13.7-10,000pg/mL
• Sensitivity: < 5.1pg/mL

• Applicable Applications for Rac1 clia kit: CLIA (Chemiluminescence Immunoassay)
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rac1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rac1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for Rac1 clia kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Rac1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Rac1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Rac1 level in the sample or standard.

Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of Rac1 in human tissue homogenates, cell lysates and other biological fluids.

Product Categories/Family for Rac1 clia kit

Signal transduction; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 51702787
• NCBI GeneID: 5879
• UniProt Accession #: P63000; O95501; P15154; Q3Y4D3; Q5JAA8; Q9BTB4; AAB22206
• Molecular Weight: 192
• NCBI Official Full Name: Ras-related C3 botulinum toxin substrate 1
• NCBI Official Synonym Full Names: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
• NCBI Official Symbol: RAC1
• NCBI Official Synonym Symbols: MIG5; Rac-1; TC-25; p21-Rac1
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 1; ras-like protein TC25; cell migration-inducing gene 5 protein
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 1
• UniProt Gene Name: RAC1
• UniProt Synonym Gene Names: TC25
• UniProt Entry Name: RAC1_HUMAN