Human 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta-1, PLCB1 ELISA Kit | PLCB1 elisa kit
Human 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta-1, PLCB1 ELISA Kit
Gene Names
PLCB1; PLC-I; EIEE12; PI-PLC; PLC154; PLCB1A; PLCB1B; PLC-154; PLC-beta-1
Reactivity
Human
Synonyms
1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta-1, PLCB1; N/A; Human 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta-1, PLCB1 ELISA Kit; PLCB1 elisa kit
Reactivity
Human
Sequence Length
1173
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
20-6000ng/L
Sensitivity
9.75ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for PLCB1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1 (also known as PLCB1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PLCB1 antibody. PLCB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PLCB1 Antibody is added and binds to PLCB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PLCB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PLCB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PLCB1 antibody. PLCB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PLCB1 Antibody is added and binds to PLCB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PLCB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PLCB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform b
NCBI Official Synonym Full Names
phospholipase C beta 1
NCBI Official Symbol
PLCB1
NCBI Official Synonym Symbols
PLC-I; EIEE12; PI-PLC; PLC154; PLCB1A; PLCB1B; PLC-154; PLC-beta-1
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
UniProt Gene Name
PLCB1
UniProt Synonym Gene Names
KIAA0581; PLC-I; PLC-beta-1
UniProt Entry Name
PLCB1_HUMAN