Standard Curve (Sample)
Human 2, 5-OAS (2, 5-oligoadenylate synthetase) ELISA Kit | 2, 5-OAS elisa kit
Human 2, 5-OAS (2, 5-oligoadenylate synthetase) ELISA Kit
Gene Names
OAS3; p100; p100OAS
Reactivity
Human
Synonyms
2; 5-OAS (2; 5-oligoadenylate synthetase); Human 2; 5-oligoadenylate synthetase) ELISA Kit; 5-OAS elisa kit
Reactivity
Human
Specificity
Specifically recognize 2, 5-OAS, no obvious cross reaction with other analogues
Sequence Length
1087
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
0.469-30ng/ml
Sensitivity
0.281ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 2, 5- OAS were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 2, 5- OAS were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for 2, 5-OAS elisa kit
Background: 2'-5'-oligoadenylate synthetase 1 (OAS1) is an antiviral protein induced by type 1 interferon that plays a key role in the cellular innate immune response. The OAS1 enzyme produces the second messenger 2'-5'-linked oligoadenylate in response to cytosolic dsRNA. These 2'-5'-linked oligoadenylates bind to the ribonuclease RNase L, which then degrades viral and cellular RNA. Polymorphisms in the corresponding OAS1 gene have been examined for possible association with increased susceptibility to type 1 diabetes mellitus, multiple sclerosis, and infection by viral pathogens.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti 2, 5-OAS antibody was pre-coated onto the 96-well plate. The biotin conjugated anti 2, 5-OAS antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with 2, 5-OAS conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of 2, 5-OAS in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti 2, 5-OAS antibody was pre-coated onto the 96-well plate. The biotin conjugated anti 2, 5-OAS antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with 2, 5-OAS conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of 2, 5-OAS in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
121,170 Da
NCBI Official Full Name
2'-5'-oligoadenylate synthase 3
NCBI Official Synonym Full Names
2'-5'-oligoadenylate synthetase 3
NCBI Official Symbol
OAS3
NCBI Official Synonym Symbols
p100; p100OAS
NCBI Protein Information
2'-5'-oligoadenylate synthase 3
UniProt Protein Name
2'-5'-oligoadenylate synthase 3
UniProt Gene Name
OAS3
UniProt Synonym Gene Names
(2-5')oligo(A) synthase 3; 2-5A synthase 3; p100OAS
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Product Notes
The Human 2, 5-OAS oas3 (Catalog #AAA27520) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA27520 ELISA Kit recognizes Human 2, 5-OAS. It is sometimes possible for the material contained within the vial of "2, 5-OAS (2, 5-oligoadenylate synthetase), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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