Standard Curve (Sample)
(Fig.1. Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) Standard Curve.)
Human 26S Proteasome Non-ATPase Regulatory Subunit 10 (PSMD10) ELISA Kit | PSMD10 elisa kit
Human 26S Proteasome Non-ATPase Regulatory Subunit 10 (PSMD10) ELISA Kit
Gene Names
PSMD10; p28; p28(GANK); dJ889N15.2
Reactivity
Human
Synonyms
26S Proteasome Non-ATPase Regulatory Subunit 10 (PSMD10); N/A; Human 26S Proteasome Non-ATPase Regulatory Subunit 10 (PSMD10) ELISA Kit; PSMD10; RP5-889N15.3; dJ889N15.2; p28; 26S proteasome non-ATPase regulatory subunit 10; 26S proteasome regulatory subunit p28; ankyrin repeat protein; gankyrin; hepatocellular carcinoma-associated protein p28-I; PSMD10 elisa kit
Reactivity
Human
Specificity
Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSMD10. No significant cross-reactivity or interference between Human PSMD10 and analogues was observed.
Sequence Length
226
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) Standard Curve.)
Related Product Information for PSMD10 elisa kit
Description: This Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMD10 in samples. An antibody specific for PSMD10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PSMD10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMD10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMD10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMD10 encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.The predicted 226-amino acid human p28 protein contains 5 ankyrin repeats, which are thought to function in protein-protein interactions.
Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMD10 encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.The predicted 226-amino acid human p28 protein contains 5 ankyrin repeats, which are thought to function in protein-protein interactions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
26S proteasome non-ATPase regulatory subunit 10
NCBI Official Synonym Full Names
proteasome 26S subunit, non-ATPase 10
NCBI Official Symbol
PSMD10
NCBI Official Synonym Symbols
p28; p28(GANK); dJ889N15.2
NCBI Protein Information
26S proteasome non-ATPase regulatory subunit 10
UniProt Protein Name
26S proteasome non-ATPase regulatory subunit 10
UniProt Gene Name
PSMD10
UniProt Entry Name
PSD10_HUMAN
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Product Notes
The Human PSMD10 psmd10 (Catalog #AAA331262) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331262 ELISA Kit recognizes Human PSMD10. It is sometimes possible for the material contained within the vial of "26S Proteasome Non-ATPase Regulatory Subunit 10 (PSMD10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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