Standard Curve (Sample)
(Fig.1. Human Actin, gamma-enteric smooth muscle (ACTG2) Standard Curve.)
Human Actin, gamma-Enteric Smooth Muscle (ACTG2) ELISA Kit | ACTG2 elisa kit
Human Actin, gamma-Enteric Smooth Muscle (ACTG2) ELISA Kit
Gene Names
ACTG2; ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
Reactivity
Human
Synonyms
Actin, gamma-Enteric Smooth Muscle (ACTG2); N/A; Human Actin, gamma-Enteric Smooth Muscle (ACTG2) ELISA Kit; ACTG2; ACT; ACTA3; ACTE; ACTL3; ACTSG; OTTHUMP00000202509; actin; gamma 2; actin-like protein; alpha-actin 3; smooth muscle gamma actin; ACTG2 elisa kit
Reactivity
Human
Specificity
Human Actin, gamma-enteric smooth muscle (ACTG2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACTG2. No significant cross-reactivity or interference between Human ACTG2 and analogues was observed.
Sequence Length
333
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Actin, gamma-enteric smooth muscle (ACTG2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Actin, gamma-enteric smooth muscle (ACTG2) Standard Curve.)
Related Product Information for ACTG2 elisa kit
Description: This Human Actin, gamma-enteric smooth muscle (ACTG2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTG2 in samples. An antibody specific for ACTG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACTG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Actin, gamma-enteric smooth muscle is a protein encoded by the ACTG2 gene. Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 2, encoded by this gene, is a smooth muscle actin found in enteric tissues.
Background: Actin, gamma-enteric smooth muscle is a protein encoded by the ACTG2 gene. Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 2, encoded by this gene, is a smooth muscle actin found in enteric tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
actin, gamma-enteric smooth muscle isoform 2
NCBI Official Synonym Full Names
actin gamma 2, smooth muscle
NCBI Official Symbol
ACTG2
NCBI Official Synonym Symbols
ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
NCBI Protein Information
actin, gamma-enteric smooth muscle
UniProt Protein Name
Actin, gamma-enteric smooth muscle
UniProt Gene Name
ACTG2
UniProt Synonym Gene Names
ACTA3; ACTL3; ACTSG
UniProt Entry Name
ACTH_HUMAN
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Product Notes
The Human ACTG2 actg2 (Catalog #AAA331085) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331085 ELISA Kit recognizes Human ACTG2. It is sometimes possible for the material contained within the vial of "Actin, gamma-Enteric Smooth Muscle (ACTG2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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